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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Objective: To investigate, 1 whether the body fluid of Ascaris lumbricoides could induce apoptosis of human alveolar epithelial cells (A549 cells) in vitro, and if there would be a possible relationship of ABF concentration or inducing time with the extent of apoptosis; 2 if the expression of bcl-2/bax would involve in and how it changes during the inducement of the apoptosis of A549 cells.

目的：用人蛔虫体腔液(Ascaris body fluid，简称ABF)体外诱导人肺上皮细胞株(A549)，观察其是否能够诱导靶细胞凋亡及凋亡与ABF浓度和作用时间的相互关系；运用RT-PCR方法检测靶细胞诱导过程中bcl-2／bax基因的表达变化，探讨其凋亡与基因表达的相互关系。

In a marriage, if one betrayed another in physical body,it is reparable; if one betrayed another in the soul, it is bound to be sad; if one betrayed another in body and soul,it will be hopeless completely.

一段婚姻中，如果一方肉体背叛了，那还有挽回的余地；如果灵魂背叛了，那这段婚姻注定是可悲的；如果肉体和灵魂都背叛了，那这段婚姻便无可救药了。

Undernutrition may be due to a failure of food supply or intake, to deliberate fasting, or to disease and is characterised by weight loss and changes in body composition, which include loss of body fat, loss of lean mass (proportionately greater in disease compared to starvation alone) and a relative increase in extracellular fluid volume.

营养不良可能是由于食品供应或摄入失败，审议禁食，或疾病，是由减肥和身体组成，其中包括身体脂肪，精益质量损失变化的特点（在疾病的比例更比饥饿单）和在细胞外液量相对增加。

METHODS: The pregnant mice were administered by oral gavage with MXC at different doses of 0, 20, 100 and 200 mg/kg during day 12-17 of pregnancy. Meanwhile, other pregnant mice as a control group were gavaged sesame oil solvent in the same manner. The changes in body mass and number of corpora lutea of the pregnant mice were observed; and the body mass and anogenital distance of their F1 foetuses were recorded, too. The concentrations of estradiol (E2 ) and progesterone in sera of the pregnant mice were determined using radioimmunoassay.

将交配成功的雌鼠于孕12～17 d每日给予不同剂量的MXC(0，20，100，200 mg/kg)灌胃，并设芝麻油溶剂为对照组，观察各组孕鼠体质量和黄体等计数测定，同时观察各组F1子代仔鼠的体质量和肛门生殖器距离等，采用放射免疫法检测孕鼠血清雌二醇(E2)和孕酮含量。

Dry processed coffees are responsible for the attractive crema on the cup, among other mechanical factors in the extraction process. Wet-processed Central Americans add positive aromatic qualities. Robustas, or coffea canephora, are used in cheaper blends to increase body and produce crema and in a few decent blends.

干处理的咖啡豆是为了生产出吸引人的crema，水洗式的中美豆增加吸引人的香味，罗伯斯特或者肯尼亚豆则是用在一些便宜的混合豆里，用来增加body和crema。

There were not correlation between the inter-colony agonistic behavior and the secretion of frontal gland, gnathal gland and abdominal gland, or the compounds of body wall, the genetic background, the contents of microelement and protein in body, the microorgansim in intestinal cavity. The colony age and the colony individuals quantity had not effects on the inter-colony agonistic behavior. But the season change, the food kinds, the soil around colony, and the weak volatile compounds from termites would influence the agonstic behavior between colonies in a certain extent.

巢间个体的格斗行为与额腺、颚腺和腹腺分泌物组成、表皮化合物组成、遗传背景、体内微量元素和蛋白质含量及体内肠道微生物的存在与否无关，巢龄和巢群大小对格斗行为也无影响，但季节变化、食物种类、供饲土壤和体表挥发物对格斗行为有一定的影响。

In order to facilitate the feasibility study of large-scale public construction, this research applies the Project Management Body of Knowledge developed by the Project Management Institute, along with the Taipei City Government's experiences, trying to conduct the management model of public construction feasibility study. This research concerns five primary processes including initialing, planning, purchasing, contract-administrating, closing. Moreover, it clearly portrays the essentials in feasibility study and organizes relevant working forms, in order to improve the public project benefit and guarantee that the public construction can be executed successfully.

为免影响国家长期民生经济发展之重要大型公共工程专案於办理可行性研究的关键阶段产生窒碍，本研究引用美国专案管理学会（Project Management Institute，PMI）之专案管理知识体系（Project Management Body of Knowledge，PMBOK）作为蓝本，以台北市政府实务上执行为例，对公共工程的可行性研究拟订起始、规划、采购、履约管理、结束等五大过程群，尝试建构公共工程可行性研究的管理模式，其中就可行性研究内的相关过程加以明确说明，并以主办机关立场概拟相关工作表单，期以提升公共工程效益，确保公共工程顺利推动执行。

The results were as follows. In the insert mutation genotypes, there was significant difference between body weights of 2 weeks aged BB and AB genotype (P.05), while there was no significant difference between AA and other genotypes. In the mutation genotypes, there was no significant difference in body weight. Insert mutation loci had no significant effect on fossil bone length and shank length of 0-12 weeks aged chickens, only mutation loci had a significant effect on fossil bone length and shank length of 6 weeks aged chickens.

分析多态位点与0-12周龄鸡生长发育性状的关联性，结果表明：插入突变基因型中，2周龄鸡体重BB型与AB型差异显著(P.05)，但AA型与AB型和BB型差异均不显著，而突变各基因型间没有显著差异；插入突变位点对0-12周龄鸡的龙骨长、胫骨长均没有显著影响，而突变位点仅在鸡6周龄时表现出与龙骨长、胫骨长间的差异显著性。

Limoleic acid found in a goji berry notably promotes loss in body fat and supports increases in lean body mass.

limoleic酸发现，在吉宝特别损失促进人体脂肪和支持增加瘦体重。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。