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The recoveries for 2,3-BD and acetoin were 91%and 92%,respectively.Aqueous two-phase extraction of 2,3-BD from real fermentation broth,the removal ratio of cells was above 99%,and the soluble proteins were about 85%in the top phase,which resulted in easy recovery of 2,3-BD from the top phase by vaporization because a viscous slurry was not formed by glucose,cells and soluble proteins at high temperature as in traditional separation methods.In addition,the cells recovered from the fermentation broth could be used as inoculum for further fermentation.

结果表明，目标产物2，3-丁二醇和乙偶姻主要富集在上相中，而葡萄糖主要富集在下相中；当异丙醇和硫酸铵的质量浓度分别是34%和20%时，2，3-丁二醇和乙偶姻的分配系数分别为8.29和8.98，回收率分别达到91%和92%；采用该条件对发酵液直接进行萃取，细胞的去除率在99%以上，可溶性蛋白去除率可达85%。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Albi also studied the discord in family relationships, In the "fragile balance", he reflected the United States a vanity of the middle of family life, his family members with each other to the shield, and do not compromise In addition, Albi is also engaged in a number of symbolic, philosophical discussion, such as the "Little Alice," and other works.

阿尔比还研究不睦的家庭关系，在《脆弱的平衡》中，他反映了美国一个中层家庭生活的空虚无聊，其家庭成员之间互相予盾、互不妥协此外，阿尔比也在进行着一些象征性、哲学性的探讨，如《小艾丽丝》等作品。

4Although Hengduan Mountains Region and its surrounding regions are the diversity center of Aletris L. in East Asia, these regions are not the speciation center.(5)Some endemic species in the regions surrounding Hengduan Mountain Region are probably the new endemic ones that had formed in the neoid period. In addition , some primitive Aletris L.

4尽管横断山及其周边地区是东亚粉条儿菜属的多样化中心，但该地区很可能并不是粉条儿菜属最早的分化中心，因横断山地区周边的一些特有种可能是在晚近的时期形成的新特有种；另外，东亚粉条儿菜属一些原始的种类主要分布于我国中东部到日本一带。

In addition, the features of elemental geochemistry of all samples suggest that (1) the EarlyTriassic andesitic rocks, characterized by characteristic natures of island arc volcanics, likely erupted in the subduction setting; that (2) the generation of LateTriassic rhyolites with an affinity of Alike granite and synchronously main body granites in the Lincang batholith displaying similar geochemical features, is closely related with the postcollision or latecollision setting; that (3) although the LateTriassic basic rocks partly show island arc volcanics such as highAl contents and NbTa depletion, some of them possessing highMg contents and mostly having traceelement ratios similar to intraplate basalt, which indicates that they erupted in the postcollision rift environment.

元素地球化学特征表明，早三叠世火山岩具有典型的岛弧火山岩的特征，暗示其很可能形成于俯冲的构造环境；中三叠世晚期的流纹岩与临沧花岗岩基主体具有非常相似的地球化学特征，结合其流纹岩具有A型花岗岩属性，表明该区中三叠世火成岩形成于碰撞后的构造背景；而晚三叠世基性火山岩虽然在地球化学特征上显示出与岛弧火山岩相似的高铝和NbTa的亏损，但其中部分火山岩具有高镁和板内玄武岩的微量元素比值特征，说明这些火山岩可能形成于碰撞后裂谷环境。

In addition, use steering angle and rear wheel angle measured in hardware loop system as the inputs of virtual prototyping model, simulate the driving track in hardware in loop simulation approximatively.

另外利用硬件在环仿真试验所测得的方向盘转角和后轮转角作为ADAMS中整车虚拟样机的控制输入，近似地模拟了硬件在环仿真中车辆的行驶轨迹。

A CHI metal ware factory was established more than 30 years ago. mainly specializing in metal ware fittings and sport equipments fittings. The products of A CHI have been appraised as famous brand products in the hardware fields. Our factory mainly deals in operations such as die casting, caulking, modeling of extension and iron pipe, the desing and development of archaistic modern products. In addition we can manufacture different atyles of hardware outer pack. Personspecially is assigned for the products in our factory.

记定五金厂成立至今已有三十余年，主要从事家具五金配件和运动器材配件，二十多年来，所开发及生产的项目得到了无数家具厂的认同和肯定，所以在五金行业中一直颇具影响，公司主要经营的项目有：压铸、冲压、铁线和铁管的造形，还有「仿古和现代」这两大类别的设计和生产，另可生产各种不同款的五金包，每款项目均有专人专业处理。

However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上，在实验室、温室和田间开展了系列研究，比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性，测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

"The company is rapidly increasing its activities around the globe," describes Berend Bracht, president and chief executive officer of Bosch Rexroth in North America,"In addition to new facilities in North and South America, Bosch Rexroth has added production capacity in Asia and Eastern Europe."

博世力士乐北美总裁兼首席执行官Berend Bracht表示：&公司正在全球范围内快速地增加部署，除了北美和南美的新工厂之外，博世力士乐还在亚洲和东欧加大了生产能力。&

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷