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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Division I is a total investment amounting to 5.9 million U.S. dollars and registered capital of 3.3 million U.S. dollars, plant area is estimated to reach over 20,000 square meters (new plant is due to the preparatory phase, the amount of future investment and equipment will greatly increase), the existing machinery Taiwan's three dozen equipment, such as: Bao Xian, network analyzers, automated assembly machines, grinders, milling machines and let Motors, riveters…… and so on, I spent Corporation, the Secretary for extension of the production technology, the introduction of advanced production equipment , The company set up more than a year ago, after product development and exploration, through advanced production equipment and technology, good quality system used to provide customers with high-quality products, particularly products of precision, stability, reliability, etc., Identified by customers in the industry are in a leading position.

我司现总投资金额为590万美元,注册资金为330万美元,厂房面积预计达20000多平方米(因新厂房正在筹备阶段,未来投资金额和设备将会大大增加),现有各种机器设备三十几台,如:剥线机、网络分析仪、自动装配机、磨床、铣床、放电机、铆钉机……等,我司延用了总公司的生产技术,引进了先进的生产设备,公司成立一年多来,经过产品不断的开发和探索,通过先进生产设备及技术,良好的质量体系运用,提供给客户高质量的产品,特别是产品的精密性、稳定性、可靠性等方面,经客户认定在同行业中处于领先地位。

In order to adapt to ceramics, pharmaceuticals, feed, metallurgy, construction, plastics, textiles, flour, cement, wood products industry's needs, I developed high-tech Secretary JS Series automatic dust collector for dust sources all have a set dust, a significant, is widely used in China in a leading position, and won the national patents, with independent intellectual property rights.

为了适应陶瓷、制药、饲料、冶金、建筑、塑料、纺织、面粉、水泥、木制品等工业的需要,我司研制的高科技JS系列全自动集尘器,用于所有产生粉尘源的集尘,效果显著,应用十分广泛,在国内处于领先地位,并获国家专利,拥有自主知识产权。

Due to the civil war caused by rebel leader Charles Taylor who has just been toppled by another rebel group and my father's status as a civil-servant by law in my country and the sensitivity of my father's position that attracts public attention, he cannot operate a Domiciliary account in order to keep this large sum of money in a bank in my country.

由于内战造成的,由叛军领袖查尔斯泰勒的人刚刚被推翻,由另一个叛乱集团和me父亲的地位,作为一个公务员的公仆依法在me的国家和灵敏度为me父亲的立场,即吸引啦公众的注意力,她也不能操作家居帐户,以保持这大笔的钱在银行,在me的国家。

Arguably, the nozzle to limit position paper, as soon as possible, and it's jitter destroyed the suction nozzle swing movement and the co-ordination of the aliquation valve, causing the pendulum back through the nozzle, the paper could not be immediately spoiledour suction nozzle feed to end just swing pressure wheel has come down upon the relationship between the paper over, resulting in a swayig press paper round has not yet been pressed paper, paper suction nozzle the cardboard in place, and with live paerboard to move back a little bit, causing a pause.

按理说,吸嘴摆到极限场所答当即不收纸,而这种抖不静爱护了吸嘴摆不静不静息与呼气阀的打扰,使得吸嘴往回摆时,未能当即不收纸,爱护了吸嘴送纸到尽脚时摆不静压纸轮恰好压住纸的移交干系,导致摆不静压纸轮还来压住纸,递纸吸嘴不离已把纸板送到位,并带住纸板此后定一下,引起迟滞。

The mousetrap must contain a solid base to attach the four other parts to, a hammer that clamps down on the mouse, a spring which gives the hammer the necessary power, a holding bar which holds the now energized hammer in position, and a catch to which the holding bar is secured, holding the hammer in coiled tension.

一个捕鼠器必须有一个坚固的底部,用来安装其余四个零件,包括一个击打老鼠的锤子,一个给锤子足够力度的弹簧,一支用来承托上了弹簧的锤子的竿,和一个用来稳定这支竿的把手,使得锤子稳定在弹簧的拉力下。

Cut file, sawing skills by learning the grip, standing position, body movements, and many other points force factors and the impact of the traditional grip of the emergence of file-grip handle to stand up after slightly outside the site of the palm, when a file cut Force, after the file handle a gradual shift,"hold" to "grasp", resulting in the introduction file enough; saw grip handle full point higher than the thrust of the saw blade to install center hole of about 30mm, the high point of thrust is likely to result in the bow saw On both sides of the swing, resulting in a broken saw blade and saw seam askew.

锉削、锯削技能的学习是受握法、站立部位、身体动作、作用力点等诸多因素的制约和影响的,传统的握法出现握锉柄后顶住部位稍偏离手心,锉削时一用力,锉柄逐渐后移,&握&变成&抓&,造成锉刀推出力度不足;满握锯柄的推力点高于锯条安装孔中心约30mm,偏高的推力点很容易造成锯弓向两侧摆动,造成锯条折断及锯缝歪斜。

Store opening and closing documents on transportation should be at the closing device, and also should do the following: a valve flap should be fixed in the open position b path ends with a foam board to plug in port, the port to be plugged with a boring cover, to dust, rust, clean and maintain the channel end of the formation; c cylinder parts to the appropriate protective packaging, shock and anti-collision; d valve should be placed on a smooth, pneumatic drive upwards, not with each other by the crowd.

存放月运输时启闭件应处于关闭装置,同时应做好以下工作: a 阀瓣应固定于开启位置 b 通径两端内口用泡沫板堵塞,端口须用闷盖塞紧,予以防尘、防锈,保持通道清洁和端面的平整; c 汽缸部位进行适当的包装防护、防震和防碰; d 阀门放置时应平稳,气动驱动装置朝上,不得相互受挤。

In this remarkable debut, a range of characters rich in practical intelligence demonstrate the importance of influence. An electrician burdened with 12 daughters persuades his employer to give him a motorcycle; a servant sleeps her way into maintaining her position in a Lahore household; a down-at-heel woman pleads for a post with a distant rich relation.

故事中务实的主角闪亮登场,娓娓道来权势的重量:担负着12个女儿生活的电工终于说服了老板,为的只是一辆摩托车;女佣不惜以身体为代价,为的只是能在拉合尔的主人家留有一席之地;衣衫褴褛的妇女恳求有钱的远亲,为的只是区区一个工作。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。