查询词典 implicit differentiation

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

To further investigate their roles in cell proliferation apoptosis and differentiation, three human leukemia cell lines (K562, U937 and HL60) exposed to various concentration of hexamethylene bisacetamide, a potent inducer of differentiation of transformed cells both in vivo and in vitro, were used as an in vitro model for the study of cell proliferation, differentiation and apoptosis.

为了进一步研究cyclin A和p21在白血病细胞增殖，分化，凋亡中的作用，我们用在体内，外均有抗肿瘤作用的药物六亚甲基二乙酰胺在体外抑制白血病细胞株K562，U937和HL60增殖，诱导其分化，成功地建立了与药物干预浓度呈剂量-效应关系的，具有不同增殖，分化，凋亡状况的实验细胞模型。

TSPG has the similar effect as HGFs, can directly or/and indirected effect and regulate proliferation and differentiation of CD34〓 HSC/HPC to bring into full play in"invigorating qi"of Panax ginseng. 2. During the process of the extensive proliferation, orderly differentiation, functional activation and gene transfer of hematopoietic stem/progenitor cells in ex vivo culture systems, TSPG may be used to regulate the proliferation and differentiation of hematopoietic cells to perform potential application in the domain of hematpoietic stem cell engineering.

我们的研究结果提示：1、TSPG具有类似造血生长因子的功效，能直接或间接影响和调控造血干/祖细胞的增殖与分化，促进血细胞的生成，从而发挥人参&补气生血&之功效。2、在进行对造血干/祖细胞的扩增与诱导分化，功能激活，基因转染等造血干细胞工程研究中可利用人参皂甙的药理作用来调控细胞的增殖分化，发挥其在造血干细胞工程领域的潜在应用价值。

Had rarely or never used protective;(11) MiceGrayAuto had realized the automatic processing of video data from the sexual preference observation system of rats, and MiceGrayManu had accomplished the analysis of images of sexual behaviors of rats;(12) Peripheral given of high dose androgen to female rats before its brain sexual differentiation had caused masculinization and defemininization in female rats, therefore led to homosexuality, and based on the results, we had established the female rat homosexual model;(13) Peripheral given of estrogen to male rats before its brain sexual differentiation had little effects on the sexual preference and sexual behavior of adult rats;(14) Peripheral given of estrogen antagonist to male rats before its brain sexual differentiation had markedly reduced masculine sexual behavior and interfered male sexual preference, but had no effect on feminine sexual behavior, and based on such results, we had established the male rat homosexual model, however the model have to be improved for better sensitivity;(15) The volume of sexually dimorphism nucleus of the preoptic area of the high dose androgen-treated female rats was significantly greater, while on the other hand the volume of AVPV of which was significantly lesser, than the female rat control.

并且有17.79％的男性同性恋者在1年内与异性保持着性关系。但在与异性阴道性交时，绝大多数（92.51％）偶然或不使用安全套；(11)MiceGrayAuto软件实现了大鼠性偏爱视频数据自动处理功能，MiceGrayManu软件实验了大鼠性行为图象分析功能；(12)大脑性分化前高剂量的雄激素有促雌鼠雄性化和去雌性化的作用，并表现为同性性倾向，建立了雌鼠同性恋模型；(13)在雄鼠大脑性分化前外周给予雌激素对成年后的性偏爱和性行为未产生明显影响；(14)在雄鼠大脑性分化前外周给予雌激素拮抗剂明显削弱雄性性行为，阻碍了雄性性偏爱，但雌性行为无明显变化，雄鼠的同性恋模型还有待进一步调整和改进：(15)高剂量雄激素处理的雌鼠SDN-POA体积明显大于对照雌鼠组，而AVPV体积明显小于对照雌鼠组。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

There are some problems in developing microcosmic syndrome differentiation, such as lack of standard for syndrome differentiation, contradiction between single microcosmic index and the entirety, nonspecificity of the relationship between microcosmic indexes and syndromes, and the influence of disease on microcosmic syndrome differentiation, discrepancy between the essence study of TCM syndromes and the clinical practice.

微观辨证&有其优越性和局限性，&微观辨证&的发展尚存在一些问题，诸如：中医证候尚未规范化、微观指标单一性与整体性的矛盾、微观指标与证候对应的非特异性、疾病对&微观辨证&产生的影响、&证本质研究与临床治疗脱节等。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用，探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用，研究毛囊上皮细胞的分化特性，我们利用团块状的毛乳头细胞，游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶，表面接种皮肤角质形成细胞或毛囊上皮细胞，进行气-液界面培养，在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

The present study demonstrates that caffeic acid phenethyl ester, a major component of honeybee propolis, enhanced ATRA-induced granulocytic differentiation in HL-60, a human promyelocytic cell line. The differentiation was assessed by Wright-Giemsa stain, nitroblue tetrazolium reduction, and membrane differentiation marker CD11b.

经由实验结果发现，利用化学染剂(Wright-Giemsa stain)观察型态， CAPE也会促进低剂量 ATRA (1nM)分化的效用，而此种分化经由 NBT reduction，和透过流式细胞仪分析分化标的膜蛋白 CD11b/CD14及细胞周期测试后，证实细胞周期停滞 G1 phase与分化现象的机制有关；再藉由免疫沉降法测试，更证实细胞周期停滞将影响 CDK2的活性。

Based on the genetic distance of 26% by SSR data, the cluster analysis with un-weighted pair-group method using arithmetic averages produced 2 major clusters: cluster Ⅰ of higher degrees of genetic differentiation and Cluster Ⅱ of lower degrees of genetic differentiation relatively, which are composed of 30 cultivars, and another cluster of much higher degrees of genetic differentiation, which is only composed of the cultivars of FHIA 25 and Pisang Jari Buaya.

依据26%的遗传距离，除了FHIA25和Pisang Jari Buaya单独化成1组外，其它30个品种可以分为2组：品种间遗传差异相对较高的组Ⅰ和品种间遗传差异相对较低的组Ⅱ。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。