查询词典 immunochemistry

Methods Immunochemistry was used to detect the IL-6 protein expression in IL-6,IL-6R and gp130 mRNA.

摘 要：目的探讨白细胞介素-6（IL-6）受体系统在肾癌细胞系RCC-949中表达的意义。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Methods:Control trial was adopted in the experiment design.Immunochemistry and RT-PCR were used to detect the expression of MMP-7 in nasopharyngeal carcinoma and chronic nasopharyngitis.

采用成组设计的对照试验方法，运用免疫组化及逆转录聚合酶链式反应技术，检测MMP-7蛋白及mRNA在鼻咽癌及慢性鼻咽炎组织中的表达。

Methods Ultrasensitive immunoassay (rate nephelometry with the Beckman Immunochemistry Systems) was used to measure...

用速率散射比浊法测定急性冠脉综合征患者和对照组血清CRP浓度，同时检测冠心病的常见危险因子并与CRP一起作Lo gistic回归分析。

Immunochemistry showed that from 2 hours through 1 week after operation, the expression of nestin was gradually enhanced in the model group in comparison with the sham group.

免疫荧光结果显示术后2h~3周，模型组nestin在Müller细胞上表达的荧光强度逐渐增强，与假手术组比较差异有统计学意义。

MethodsAfter fixed by 4% paraformaldehyde and embedded in paraffin,retinas of 26 healthy adult Wistar rats were studied by immunochemistry and digital photomicrograph analysis to detect the locations and levels of the expression of SDF-1 and pan cytokeratin.

本研究的主要目的在于了解生理状况下SDF-1在成年大鼠视网膜上的表达情况，为眼内新生血管

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

The NO content and the NOS activity were down in the PIH pregnant women placenta.This showed that the ghrelin regulated the VEGF and influenced the the NO down.And the placenta angiospasm,blood pressure highten, and the PIH were caused also by this. The ghrelin,VEGF,Flt-1,NO and NOS were detected by the SP-immunochemistry, RQ-PCR,cell culture in vitro,ASODN and nitrate reductase in the normal pregnancy,PIH placenta and the cultured HUVEC antisense blocking ghrelin in vitro.

结果证实：PIH孕妇胎盘组织中Ghrelin，VEGF、Flt-1蛋白和mRNA水平下降与正常孕妇比较有显著性差异p＜0.05提示在PIH发病过程中Ghrelin可能对VEGF，Flt-1蛋白和mRNA的表达具有下调作用，进而影响胎盘血管网的形成和发育；NO含量与NOS活力水平在PIH孕妇胎盘组织中下降，提示Ghrelin可能通过下调VEGF的表达进一步影响血管舒张因子NO水平下降，进而引起胎盘血管痉挛，血压升高，最终导致PIH的发生。

Methods: 30 adul male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle with coriaria lactone (50g/kg). The sections of the brains were processed by immunochemistry with antibodies against SYN and GFAP.

成年健康雄性SD大鼠30只，随机分为模型组及对照组，采用马桑内酯(50g/kg)侧脑室注射法建立急性癫痫动物模型，应用免疫组织化学方法观察癫痫持续状态下SYN和GFAP的表达。

In our stydy, we explored the expression pattern of COX-2 in normal rat Pilosebaceous Unit and hair follicle cycle adopting immunochemistry and RT-PCR methods.

毛囊皮脂腺单位中COX-2仅在表皮基底上层细胞和皮脂腺中，而在毛囊各层细胞中基本未见表达(仅在特化的触须毛囊外根鞘有轻微表达)。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。