查询词典 immunoassay

Compared with conventional methods, this method is sensitive for the selective immunoassay in lower strength of biological sample and quantitative analysis.

它不仅能弥补目前常规免疫分析方法不能定量的不足，而且能实时监测免疫反应全过程，把反应信号连续地记录下来。

Objective A bioluminescence - based immunoassay was established for the quantitation of PCR products.

目的基于水母发光蛋白生物发光分析技术建立一种PCR产物定量检测技术。

Methods:the quantitative ultrasonography of calcaneum was tested in Tajiquan group,the aged group and the adult group and by enzyme immunoassay,the concentrations of T FT DGT AND SHBG were measured in the study.

通过测定太极拳组、老年对照组、成年对照组的跟骨超声参数及血清男性激素水平。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

Different aspects associating the DMAE-NHS-based CLIA were studied in this thesis,including synthesis of acridinium ester,chemiluminescent characteristics of DMAE-NHS,labelling antibody or streptavidin with DMAE-NHS,two-site sandwich chemiluminescent immunoassay for TSH,two-site sandwich chemiluminescent immunoassay involved biotin-streptavidin system for TSH,and competitive chemiluminescent immunoassay using biotin-streptavidin system for TLVThe desired acridinium ester,DMAE-NHS,was synthesized according to the reference method with some modifications.

目前国内尚未见有关DMAE·NHS的合成及其应用于CLIA的研究报导，本论文研究了吖啶酯DMAE·NHS的有机合成方法，DMAE·NHS的发光性能，DMAE·NHS及其与抗体偶联物的热稳定性，DMAE·NHS标记TSH单克隆抗体和链亲和素，TSH CLIA，TSH BAS-CLIA和TT_4 BAS-CLIA。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

Important advance was made to Celastrus and ceangulin V after 20 years studies, but the target of Celangulin V was not verified and the metabolic dynamic of Celangulin V in organism was not touched because of the technique difficulty in testing and analysis of trace Celangulin V in organism. It will solve the difficulty that establishing the immunoassay of Celangulin V. The immunoassay of Celangulin V including the synthesis of artificial antigen, the preparation of antiserum, the immunoanalysis location of Celangulin V and the quantitative immunoassay of Celangulin V were studied systematicly in this paper.

苦皮藤素V是从卫矛科植物苦皮藤中分离出的一种具有杀虫活性的天然产物，可能作用于一类新的作用靶标。20多年对苦皮藤及苦皮藤素V的研究已取得了重要进展，但迄今还没有证实苦皮藤素V的作用靶标，对苦皮藤素V在生物体内的药代动力学研究也尚未涉及，这些方面的研究因为对痕量苦皮藤素V在生物体内分析、检测存在技术方法上的困难而受到限制，建立苦皮藤素V的免疫分析技术则可解决这些问题。

Important advance was made to Celastrus and ceangulin V after 20 years studies, but the target of Celangulin V was not verified and the metabolic dynamic of Celangulin V in organism was not touched because of the technique difficulty in testing and analysis of trace Celangulin V in organism. It will solve the difficulty that establishing the immunoassay of Celangulin V. The immunoassay of Celangulin V including the synthesis of artificial antigen, the preparation of antiserum, the immunoanalysis location of Celangulin V and the quantitative immunoassay of C'elangulin V were studied systematicly in this paper.

苦皮藤素Ⅴ是从卫矛科植物苦皮藤中分离出的一种具有杀虫活性的天然产物，可能作用于一类新的作用靶标。20多年对苦皮藤及苦皮藤素Ⅴ的研究已取得了重要进展，但迄今还没有证实苦皮藤素Ⅴ的作用靶标，对苦皮藤素Ⅴ在生物体内的药代动力学研究也尚未涉及，这些方面的研究因为对痕量苦皮藤素Ⅴ在生物体内分析、检测存在技术方法上的困难而受到限制，建立苦皮藤素Ⅴ的免疫分析技术则可解决这些问题。

The SPA-rich Staphylococcus aureus coated with the specific IgG was used as nephelometric immunoassay reagent and could lead to good results. On the basis of nephelometric immunoassay combined with immunomagnetic separation, the rapid automatic quantitative immunoassay for the detection of Shigella flexneri was established, which provides a new way for the rapid diagnosis and automatic quantitative analysis of pathogenic bacteria.

以福氏志贺菌的多抗血清分别致敏SPA包被的磁珠和富含SPA的金黄色葡萄球菌，制备特异的多抗磁珠对福氏志贺菌有一定的特异捕获与富集作用，抗体致敏的金黄色葡萄球菌作为协同凝集试剂与免疫比浊试剂对福氏志贺菌的自动、快速、定量检测具有较好的效果，免疫比浊分析结合免疫磁分离技术为病原菌的快速诊断和自动定量分析提供了新的思路。

Chemiluminescence Immunoassay is an efficient detection tool combined from chemiluminescent reactions and immunoassay.

化学发光免疫技术是化学发光与免疫测定结合起来的一种高效检测手段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。