查询词典 immunized

For immunized egg laying chicken groups, cloacal swabbing samples were collected at 7 days post challenge, and no shedding was found in groups vaccinated with rFPV-H5HA-I

结果表明，rFPV-H5HA- IL18、rFPV-H5HA-H7HA-IL18免疫组在攻毒后第7d无排毒，其抑制免疫鸡排毒效果优于常规疫苗和单独表达 HA的rFPV-H5HA重组鸡痘病毒。rFPV-H5HA-IL18和rFPV-H5HA-H7HA-IL18免疫组鸡，在14日龄时的体重明显高于rFPV-H5HA免疫组和常规疫苗对照免疫组，表明共表达的鸡IL-18能降低鸡痘病毒载体对雏鸡增重的影响。

The chickens were immunized with ND attenccated vaccine by dropping cloacal, nasal, oral at 14 and 28 days old, and the control groups were set up.

采用新城疫Lasota弱毒疫苗于14日龄和28日龄通过泄殖腔、滴鼻、饮水免疫雏鸡，并设对照组。

After 10 weeks immunization, the combinant plasmids distribute different organs and tissues of the BALB/c mice immunized by DNA vaccines of Toxoplasma gondii ,but only blood was Detected out the recombinant plasmids.

结论弓形虫DNA疫苗免疫BALB／c鼠5周后，多个不同器官组织均可检测出重组质粒，免疫后10周血液检测出重组质粒。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶（基因命名为KLK1，酶命名为hK1）cDNA克隆入大肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

Establishing experimental animal model of immunized fibrosis could contribute to research on extracellular matri and cytokine. they turns out that the ingredient of Desmodium pulchellum can relieve extra growth and deposit of ECM and adjust the level of relative cytokine, such as TGF-β1 and IFN-γ, benefit to effect of hepatic anti-fibrosis.

结果证明排钱草生物碱类成分能减轻肝纤维化的基本病理改变即减少肝细胞外基质的过度增生和沉积，调节与肝纤维化密切相关的TGF-β1、IFN-γ等细胞因子水平，向着有利于减轻肝纤维化的方向发展。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Healthy dogs were inoculated with rabies vaccine and antibody titre was measured by ELISA and FAVN method from the 14th day. The immunized dogs were challenged with rabies virus BD06 strain.

将40只犬免疫兽用狂犬病疫苗后，第14 d静脉采血分离血清，分别采用ELISA法和国际贸易指定试验FAVN法检测血清样品，试验犬免疫第21 d攻击狂犬病毒BD06株。

This study aims at tick vitellogenesis and its mechanism. Six hybridoma cell lines secreting monoclonal antibody against vitellin of the hard tick Haemaphysalis longicornis were produced by fusing myeloma cells (SP2/0) with spleen cells, both from BALB/c mouse which were immunized with the Vn. They were named as 1B5, 2A7, 2B8, 2F2, 3A1 and 3G1, respectively. Further identification indicated that 1B5, 2B8 and 2F2 were of the isotype IgGA, 2A7 was of the isotype IgG1, while 3A1 and 3G1 were of the isotype IgG2a.

为研究蜱类的卵黄发生及其机理，用长角血蜱Haemaphysalis longicornis卵黄蛋白(vitellin，Vn)免疫BALB/c小鼠，取免疫鼠脾细胞与骨髓瘤细胞SP2/0进行融合，经3次克隆化筛选，获得6株能稳定分泌抗Vn的单克隆抗体，即1B5，2A7，2B8，2F2，3A1和3G1.1B5，2B8和2F2亚型为IgGA；2A7亚型为IgG1；3A1和3G1亚型为IgG2a.6株McAb均具有高度特异性，效价在1：105以上。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。