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Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

Objective To investigate the effects of CpG oligodeoxyribonucleotide as adjuvants on cellular immune responses in mice immunized with hepatitis B virus gene vaccines.

目的 探讨人工合成的含CpG基序的寡核苷酸作为佐剂对乙型肝炎病毒基因疫苗诱导小鼠产生细胞禁忌免疫应答的影响。

Antisera from normal mice and mice immunized with pcDNA3 were both negative on Jurkat cells.

免疫组化结果表明，该血清不与表达TCR γδ的人T淋巴瘤细胞发生反应，而与转染该基因的SP2/0细胞产生强阳性反应。

Methods: BALB/C mice were immunized with Bacteroids fragilis.

目的：为了获得高效价和高特异性的抗Bf抗体。

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

female Lewis rats with 6-8 weeks of age and 160-180g of weight were immunized subcutaneously into both hind footpads with 50μg Torpedo AChR emulsified in complete Freud's adjuvant in a total volume of 200μl and divided into 4 groups at random.

ELISA法动态观察外周血抗AChR抗体IgG含量以及特异性抗体亲和力的改变。3.ELISA法测定致敏后第45日，大鼠血清中AChR特异性IgG抗体亚型IgG1、IgG2a、IgG2b含量的改变。4。

In the first experiment, C57BL/6J mice (6weeks, females) was pre-immunized with the normal rabbit IgG in rear footpads. Five days later, mice were received intravenously injection with 100, 150 and 200ml of NTS, respectively. Control mice were treated for normal rabbit serum.

诱发肾炎实验以6周龄雌鼠(C57BL/6J mice)为宿主，先在小鼠足底皮下注射正常兔子之IgG；5日后实验组分三组分别尾静脉注射肾毒血清NTS (100ml， 150ml， 200ml)，对照组则给予正常兔子血清。

Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated. A series of generic haptens were synthesized and coupled to bovine serum albumin and ovalbumin for immunogens and coating antigens, respectively. New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.

通过分析硫代磷酸二乙酯类农药的结构特点，设计并合成了系列半抗原；采用活泼酯法将半抗原分别与牛血清蛋白和卵清蛋白偶联制备了系列免疫原和包被原；通过免疫新西兰大白兔获得了相应抗硫代磷酸二乙酯类农药的类特异性抗体。

Then, the hapten LBc and LBs was conjugated to bovine serum albumin with CDI method to form immuno-antigen; the haptens LBc, LBs and LBy was conjugated to ovalbumin with mixed anhydride method to form the the coating antigens.The new zealand rabbits were immunized by conjugate of LBc-BSA and LBs-BSA, and titres of anti-bifenthrin serum (5.12×10~4, 3.2×10~5) were determined by non-competitive indirect enzyme-linked assay procedure.

通过碳二亚胺法将LBc和LBs交联于牛血清蛋白作为免疫抗原，通过活泼酯法将分别LBc、LBs和LBy交联于卵清蛋白作为包被抗原，LBc-BSA和LBs为免疫原制备了联苯菊酯的兔抗血清，非竞争间接酶联免疫吸附分析方法测得其效价达5.12×10~4和3.2×10~5。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。