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The in vitro and in vivo activity of these specific IgY and their opsonization to phagocytes were estimated.Specific IgY were produced by hens immunized with formaldehyde killed S.aureus or E.

本研究制备了抗奶牛乳腺炎主要致病菌——金黄色葡萄球菌和大肠杆菌特异性卵黄抗体，考察了抗体的体外抑菌活性、对吞噬细胞的吞噬调理作用及对奶牛乳腺炎的治疗作用。

The recombinant protein was purified by Ni-NTA affinity chromatography and gel filtration chromatography. The inbred C57BL/6 strains and outbred KM strains of mice were subcutaneously immunized with the protein formulated by either adjuvant ISA720 or aluminium hydroxide.

表达产物通过Ni-NTA亲和层析和凝胶过滤层析方法纯化，再分别与ISA720和Al3佐剂乳化后，免疫C57BL/6和昆明小鼠，通过尾蚴膜反应和ELISA反应检测特异性抗体。

In the control animals,the GAP-43-LI nerve fibers were found mainly distributed in association with vascular plexuses,with minor extension into the parenchyma of the inner zone of the periarterial lymphocyte sheath.In the immunized animals,in addition to denser vascular plexuses,more fibers appeared in the outer zones of periarterial lymphocyte sheath,the marginal zone,and the red pulp,all known to be the sites of active immune response.

于对照动物，脾GAP-43-LI神经纤维主要分布于血管周围，少量伸入动脉周围淋巴鞘的脾基质中，而在免疫刺激的动物，不仅血管周围的神经纤维明显增多，动脉周围淋巴鞘外层、边缘区和红髓等免疫应答活跃的部位也出现许多神经纤维。

There are high level of specific IgG and IgA against Fe-SOD in sera and lavage of intestines in mice intranasally administrated with S. typhi Fe-SOD, but there are high level of specific IgG in sera only in mice immunized with S. typhi Fe-SOD peritoneally.

结果 当用IL-1作为佐剂时，经鼻腔接种伤寒杆菌Fe-SOD的小鼠血液和肠液中可产生高水平特异性IgG和IgA；而腹腔注射仅在血液中产生高水平特异性IgG。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

If these people are not immunized, and think that they could protect patients by simply not coming in to work if they are sick, they could spread influenza to patients during the presymptomatic phase of their illness.

如果这些人不接种疫苗，认为他们如果生病了只要不去上班就可保护患者不被感染，那么他们很可能在疾病症状发作前期就已经将流感传染给了患者。

We found that both SL7207 and SL7207/pEGFP-N1 vaccines can not induce theprotective immunity,while SL7207/pGFP-FL vaccines can trigger antitumorresponses in partial (2/10) of orally immunized mice.And these two tumor-free micealso got the immunity to rechallenge the 10-fold dose of H1 tumor cells when given40 days post the primary challenge.

我们发现，野生型的SL7207和SL7207/pEGFP-N1均不能诱发小鼠的保护性免疫，而SL7207/pGFP-FL则可以在部分小鼠（2/10）中诱发保护性免疫，使小鼠免生肿瘤，并能抵抗在初次荷瘤攻击后40天，10倍于首次剂量的H1肿瘤细胞的再攻击。

After restimulation in vitro with HTHV plus rhuIL-2, the splenocytes either from HTHV infected mice or from pEF-BOS/HTHV-NP plasmid DNA immunized mice were used as effectors in 4 h 51 Cr release assay.

分别取HTHV感染小鼠的脾细胞和pEF-BOS/HTHV-NP质粒DNA免疫小鼠的脾细胞，经体外抗原和IL-2再刺激诱导产生CTL效应细胞，进行4h 51 Cr释放实验。

We paralleled to study the HSV seropositive mice. Balb/c mice were immunized intraperitoneally with mtHSV and HSV-1 F.

因此，我们深入地研究了mtHSV对HSV预免疫的血清阳性Balb/c鼠的肿瘤治疗效果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。