查询词典 immunized

Claudia wanted people sneezing, getting immunized, that sort of thing, recalls Harmel, a slight, soft-spoken 52-year-old.

克劳蒂娅想要一些人们在打喷嚏、接受疫苗注射等诸如此类的照片，马克回忆道。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Pallidum-antibody with indirected-ELISA based on the fusion protein. Zealand rabbits were immunized with the fusion protein, antibodies to anti-Gpd in sera were detected with indirected ELISA.

同时用纯化的Gpd重组蛋白免疫新西兰兔，间接ELISA方法检测免疫兔血清中Gpd多克隆抗体的效价，对Gpd重组蛋白的免疫原性进行分析。

The eukaryotic expression plasmids were used as genetic vaccines to immunized intramuscularly experimental animals and post-weaning piglets.

同时还研究证明猪白细胞介素2和6基因对猪伪狂犬病核酸疫苗的免疫增效作用。

Methods New Zealand white rabbits were immunized with synthetic murine obestatin to obtain anti-serum,while chloramines-T method was used to iodinate obestatin antigen and a method of RIA for obestatin was established.

利用人工合成的鼠Obestatin免疫新西兰白兔制备抗血清，采用氯胺-T法碘标记Obestatin抗原，从而制备Obestatin RIA。

Induction of isotypic heterostrain antibody (Ab1): BALB/c mice were immunized with C57BL/6 splenocytes to raise antibody (Ab1) between the two difference strains.

抗同种异品系抗体(Ab1)的诱导 C57BL／6小鼠作为移植供体，BALB／c小鼠作为受体。取C57BL／6小鼠的脾细胞免疫BALB／c小鼠，制备抗同种异品系抗体(Ab1)。

MethodAmoxicillin were coupled with bovine serumalbuminand oval buminby using N-hydroxysuccini mide actived ester method to prepare the i mmune antigen AMX-BSA and detection antigen AMX-OVA.The adult rabbits were immunized with AMX-BSAto obtainthe polyclonal antibody with high titer.

方法采用N-羟基琥珀酰亚胺活性酯法将阿莫西林分别与牛血清白蛋白、卵清蛋白偶联，制备免疫抗原AMX-BSA和检测抗原AMX-OVA，用AMX-BSA免疫成年兔以获得高效价的多克隆抗体。

When monotocous breedof ewes were immunized with various dose of pCIS, 7 out of 27 ewes produced twin lambs.

应用各种剂量的 pCIS 免疫27只单胎品种绵羊，结果有7只产双羔(孪生率25.9%)。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

The cytotoxicity activity of the whole or nonadherent splenocytes from immunized mice was measured (Hca-F, L929 as target cells) by MTT method.

通过MTT法测定免疫小鼠全脾细胞或不粘附细胞的细胞毒活性(Hca-F、L929为靶细胞)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。