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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Objective:To investigate the dynamic changes of immune function indexes of erythrocyte in the dialysis process of patients subject to blood dialysis, and to study the contribution of dialysers with different biocompatibilities to the changes.

目的：了解血液透析病人透析过程中红细胞免疫功能指标的动态改变及不同生物相容性的透析膜对其变化的影响。方法：分别采用铜仿膜、聚砜膜、聚甲基丙烯酸甲酯膜，观察了透析过程中不同时相的红细胞免疫功能指标RBC－C3b和RBC－IC的变化。

1 The SHXLF can inhibit the invasion of the hepatocellular carcinoma in mice's footpads and lymph nodes, and it can decrease the index of lymph node, prevent lymphatic metastasis.2 Its mechanism is to improve immune functions and injury the carcinoma cell, to decrease VEGF content and MVD in liver cancer, so as to inhibit the tumor angiogenesis.

1麝黄消瘤方可抑制H_(22)肝癌小鼠癌组织在局部及淋巴结的侵袭程度，降低淋巴结指数，抑制淋巴道转移。 2其作用机制主要是通过调整免疫功能，杀伤癌细胞，抑制肿瘤血管生成而实现的。

objective to observe the protective effect of qiliqiangxin capsule in rabbit heart ventricle reconstitution of chronic heart failure.methods using the method of giving rabbit abdomen aorta half ligations to build the chronic heart failure model to observe qiliqiangxin capsule the effect to blood flow mechanics index,room wall thickness,heart exponent and heart ventricle reconstitution biochemistry index.results qiliqiangxin capsule could reduce effectively bp,lvsp,lvedp,t wave,improve room wall thickness and heart exponent,reduce angii and alt,all the paramenters ahd notable difference,composed to those of model group (p＜0.05,p＜0.01).conclusion qiliqiangxin capsule can improve the blood flow mechanics change of chronic heart failure rabbit,which has notable inhibitory action on heart ventricle reconstitution which originate from chronic heart failure.inhibition of ang ii,alt of immune nerve endocrine system may be a mechanism of qiliqiangxin capsule.

观察血流动力学、标志心室重构的室壁厚度及心脏指数及肾素―血管紧张素―醛固酮系统相关生化指标的变化。结果芪苈强心胶囊可有效降低血压、左室压、左室末期舒张压、t波，改善室壁厚度和心脏指数，降低血清血管紧张素ii及醛固酮水平，与模型组比较均有显著性差异(p＜0.05，p＜0.01)。结论芪苈强心胶囊可改善慢性心力衰竭兔血流动力学的变化，对慢性心力衰竭引起的心室重构具有显著抑制作用，其机制可能与该药发挥抑制神经内分泌免疫系统rass中angii、ald的水平有关。

This 2.5-meter-long wholegrain cake, enough to feed the more than 1,000 visitors to the show, is not only supposed to help boost your immune system, but also tastes pretty good too.

而这个足够与现场一千多人分享，长达2.5公尺的养生五榖糕点，不但有提升免疫力的功能，点缀了时令水果更是让人看了垂涎三尺，也是看展民众不会错过的焦点。

There is probably a genetic contribution, particularly in genes controlling the level of immune response – although genetic susceptibility is likely to be linked to an environmental factor, some possible factors including vaccination or exposure to silicates.

有可能是遗传的贡献，特别是在基因控制水平的免疫反应-尽管遗传易感性可能是与环境因素，一些可能的因素，包括预防接种或接触矽酸盐。

This leaves children highly sensitised to the particular allergen, which can lead to asthma attacks when their immune system reacts to any subsequent exposure.

这使得儿童对特殊致敏原高度敏感，当他们的免疫系统对以后接触到的任何致敏原起反应时都能诱发哮喘发作。

However, the ruses are so smart that they could be able to produce some chemical substances to cause our immune cells to die.

於是，我们身体当中的免疫细胞就会很快的杀掉这些感染病毒。

Currently, there are three major obstacles to performing transplantations from pig to man: a powerful immune barrier, a potential risk of transmitting microorganisms, particularly endogenous retrovirus, and ethical issues related to the future recipients and to society at large.

目前，对于实施猪到人的器官移植，有3个主要障碍：强烈的免疫排斥；跨种系感染的潜在危险，特别是内源性逆转录病毒；将来对移植受体和社会的伦理问题。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。