查询词典 immune system

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

objective to observe the protective effect of qiliqiangxin capsule in rabbit heart ventricle reconstitution of chronic heart failure.methods using the method of giving rabbit abdomen aorta half ligations to build the chronic heart failure model to observe qiliqiangxin capsule the effect to blood flow mechanics index,room wall thickness,heart exponent and heart ventricle reconstitution biochemistry index.results qiliqiangxin capsule could reduce effectively bp,lvsp,lvedp,t wave,improve room wall thickness and heart exponent,reduce angii and alt,all the paramenters ahd notable difference,composed to those of model group (p＜0.05,p＜0.01).conclusion qiliqiangxin capsule can improve the blood flow mechanics change of chronic heart failure rabbit,which has notable inhibitory action on heart ventricle reconstitution which originate from chronic heart failure.inhibition of ang ii,alt of immune nerve endocrine system may be a mechanism of qiliqiangxin capsule.

观察血流动力学、标志心室重构的室壁厚度及心脏指数及肾素―血管紧张素―醛固酮系统相关生化指标的变化。结果芪苈强心胶囊可有效降低血压、左室压、左室末期舒张压、t波，改善室壁厚度和心脏指数，降低血清血管紧张素ii及醛固酮水平，与模型组比较均有显著性差异(p＜0.05，p＜0.01)。结论芪苈强心胶囊可改善慢性心力衰竭兔血流动力学的变化，对慢性心力衰竭引起的心室重构具有显著抑制作用，其机制可能与该药发挥抑制神经内分泌免疫系统rass中angii、ald的水平有关。

TCM may modulate the glial cells through the following mechanisms: inhibiting expression of iNOS, thereby minishing the toxicity of NO and its oxygen free radicals; inhibiting overactivation of astrocytes and overexpression of GFAP in acute cerebral ischemia and cerebral hemorrhage; promoting glial cells' secretion of NGF which can be caused by ischemic injury; inhibiting the nervous system's immune inflammatory response; and so on.

中药调节神经胶质细胞活性的机制可能包括：（1）抑制iNOS表达，从而抑制NO及其产生的氧自由基的毒性；（2 抑制脑缺血和脑出血急性期星形胶质细胞的过度活化和GFAP的过度表达；(3)上调缺血损伤导致的神经胶质细胞分泌NGF；(4)抑制神经系统的免疫炎症反应等各种途径。

To investigate the expressions of Fas and FasL on tumor cells and TILs in patients with so as to understand the potential role of Fas system in immune escape and counteratteck involved in HCC,the expression levels of Fas and FasL were detected on tumor cells,noncancorous liver cells,TILs and PBLs in patients with HCC by means of indirect fluorescent immunoassay flow cytometry.

为研究肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞Fas抗原与FasL配体的表达水平，探讨Fas系统在肝细胞癌免疫逃逸及免疫反击中的作用，采用间接免疫荧光流式细胞术检测20例肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞和外周血淋巴细胞Fas与FasL的表达。

AIDS is a retrovirus infection caused by the body's immune defense system diseases.

艾滋病是一种由逆转录病毒引起的人体免疫防御系统方面的疫病。

In order to detect the integrity of migrating instance in migrating workflows system,an immune entity composed of self-set,detector-set,r-continuous bits match detection function and vaccine gene-set is defined based on artificial immunology principle.

针对迁移工作流系统中迁移实例的完整性检测，基于人工免疫原理，构造一个由自我集合、检测器集合、r-连续位匹配函数和疫苗基因集合组成的免疫体。

Comparative studies of immune functions in echinoderms have demonstrated that there is a complement system in echinoderms that appears to have alternative pathway and lectin pathway but lack classical pathway and terminal pathway.

比较免疫学分析表明，棘皮动物存在脊椎动物补体系统的替代途径和凝集素途径，但未发现经典途径和明确的终端途径。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了