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II. Exploring non-radioactivc method to select the optimal DNA-bindingsequencc fOr singIe-chain reprcssorCloning and expression of single-chain repressors with cysteine tai1 wereperformed and immobilized these protein in the solid surface by using the standardmicrowell plates Which have been activated to contain maleimide grouPs, al1owing tobind sulthydryl-containing protein on its surface.

二。非同位素的方法筛选单链阻遏蛋白的最佳DNA结合序列初探克隆和表达了带半眈氨酸尾的单链阻遏蛋白，利用己包彼了马来酚胺的活性板可以与自由琉基结合的特性，将蛋白固定在活性板表面。

Firstly, phenyliodine diacetate was immobilized to the 2% cross-linked polystyrene or the liner polystyrene resin. The more iodine the resin had, the lower conversion to phenyliodine diacetate. We made our endeavor to extend the range of useful application of polystyrene supported phenyliodine diacetate in organic synthesis:(1) the reagent was used as an effective reagent of carbonyl regeneration from oximes and semicarbazones of ketones.(2) the reagent was also applied to the synthesis of 1, 3, 4-oxadiazoles, 1, 3-disubstituted-2-pyrazoline, 2-arylbenzoxazole from aldehyde N-acylhydrazones, aldehyde hydoazones and phenolic Schiff's bases respectively.

首先，我们以2％交联的聚苯乙烯和线性的聚苯乙烯树脂为载体，制备了聚合物负载的二醋酸碘苯，通过研究发现：(1)聚苯乙烯二醋酸碘苯是酮肟、缩氨脲有效的去保护试剂，不仅芳香酮，脂肪酮和α，β不饱和酮的肟、缩氨脲也能顺利转化为相应的酮，实现酮基的再生；(2)我们还利用此试剂，分别与醛的N-酰基苯、N-酰基苯腙及邻羟基芳亚胺反应，在温和的条件下发生成环反应，以良好的产率生成1，3，4-噁二唑、吡唑啉和二取代苯并噁唑等杂环化合物；(3)聚苯乙烯二醋酸碘苯还可有效地将邻羟基芳酮的酰腙经氧化、重排成1，2-二酰基苯。

The objective of this work is to study the characterization of immobilized polyphenol oxidase from banana leaves and its application on the transformation of phenolic compounds. Polyphenol oxidases of banana leaves from a variety of sources were compared. Among them, PPO from Giant cavedishii leaves showed the highest specific activity (315 units/mg).

摘要 本研究目的在探讨固定化香蕉叶多酚氧化酶之特性及於酚类化合物转化之应用，首先探讨不同品种香蕉叶多酚氧化酶并比较不同之固定化方法，结果以北蕉的单酚酶比活性最高315，活性约为其他品种之2-20倍。

For C/N of hog manure is lower than that of straw, quick-acting nitrogen in soil of the treatment applied M F was immobilized less than that applied C or W F.

因此，有机肥与化肥配施的各个处理在玉米拔节期，必需适量追肥，以满足玉米生育后期生殖生长的需求。

The animals were randomly divided into groups as follows: testⅠ: Group S, 20 rats were immobilized by using of flexible wire mesh (5x5mm) bent to bodies, and freed of food and water during the experimental period (8pm-8am).

本研究目的是结扎建立实验性牙周炎动物模型，研究限制性应激与牙周炎之间的关系；在应激基础上给与大鼠尼古丁，探讨应激和吸烟协同作用在牙周炎的发生发展过程中的作用，为进一步阐明牙周炎的发病机制奠定基础。

31DH suspend in the dry red wine and ferment,the density of the dry immobilized 31DH was changed by various materials.

通过选用不同的材料调节干固菌胶粒的比重，使固定化酒类酒球菌31DH悬浮于干红葡萄酒中进行苹果酸—乳酸发酵，并测定这些材料对固定化胶粒相对扩散系数的影响。

In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes.

就分析的蛋白质微阵列来说，具有特定活性的很好描绘的分子，如抗体，肽-MHC复合物，或凝集素，被用作固定的探针。

But Ennis, haunted by a childhood memory of his father taking him to see the mutilated body of a rancher, tortured and beaten to death with a tire iron for living with another man, is immobilized by fear and shame.

但恩尼斯踟躇了，他记得尚是孩提时，他父亲曾带他去看过一个牧场主被肢解的残骸---因为他与另一个男人生活，而被人用轮胎铁监生打死。这让他觉得恐惧和羞耻。

The study has laid a foundation for MSFB with immobilized microorganism applied in the treatment of sulphur-containing waste water.

以上研究为固定化生物膜磁场流化床反应系统应用于含硫废水处理奠定了坚实的基础。

PVA was used as the basic carrier, and alginate sodium and silicon dioxide were added selectively to prepare complex carrier of immobilized yeast and then it was applied in liquor production by liquid fermentation.

以聚乙烯醇为基本载体，选择性地加入海藻酸钠、二氧化硅，制备成固定化酵母细胞的复合载体在液态法生产白酒中应用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。