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Tri- sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol; The chicken IgG(anti-JG04 strain GPMV) was immobilized to a defined detection zone on a porous nitrocellulose membrane, while 3A_5 was conjugated to colloidal gold which served as a detection reagent.

在加样处沾取含有GPMV的尿囊液样品，GPMV与金标3A_5结合形成混合液，由于层析原理，混合液向上移动，在检测线上混和液中与金标颗粒结合的GPMV与鸡IgG结合，由于大量GPMV沉积在检测线上，出现红线；而如果尿囊液中不含有GPMV，检测线上不出现红线。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

The results indicate that sepoilte heat-treated in air at 400℃ is the best immobilized carrier of the strain.

结果表明，活化温度为400℃的海泡石最适宜作固定化载体。

It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.

在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

Methods Glutamate decarboxylase was embedded-immobilized with sodium alginate and gelatin as the blending supports. Factors which affected the activity of IGAD were studied and optimum conditions were investigated. Afterwards, IGAD was applied for GABA transformation.

以海藻酸钠与明胶为协同作用载体包埋固定化酶，考察影响固定化酶活性的因素，获得固定化酶的优化条件，并将酶应用于制备GABA。

The traditional approach to supplementing P for plants is to apply P fertilizer to the soil, but most of it may be readily immobilized or fixed by calcium ions in the lime soil or by iron and aluminum hydroxide in the acid soil, which results in a low use efficiency of P applied.

改善植物磷营养的传统方法是对土壤增施磷肥，但大部分磷肥被石灰性土壤中的钙和酸性土壤中的铁、铝氢氧化物固定成为无效态在土壤中积累起来。

Purpose:The aim is to study the optimal co reaction condition of producing high content fructooligosaccharides for immobilized cells and glucose isomerase.

目的 ：研究固定化黑曲霉细胞与葡萄糖异构酶协同反应生产高含量低聚果糖的优化条件。

It has been indicated that antibiotic activity of lincomycin reaction solution increased twofold in after 32h reaction with immobilized protoplast of S.

用固定化金霉素产生菌（金霉素链霉菌，S.aureofaciens）的原生质体，在适当量的氯离子存在时，转化林可霉素生成抗菌活力比其本体高的产物。

Immobilized within loofa sponge were explored as a function of initial solution pH, temperature, contact time and initial metal concentration.

研究其在溶液中对金属离子Pb^2＋和Cu^2＋的吸附效果，并探讨其生物吸附机理，分析其动力学特性。

Based on the relationship and the phenomenon model, an improved model of MR damper has been presented in this paper.

摘 要：本文在实验数据的基础上建立了磁流变阻尼器输出力与不同常电流控制量的关系，对现有的 p模型进行了改进。

I don't think our founding fathers were very fuckable.

我认为我们的开国元老没什么吸引力