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A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Enzymatic interesterification of palm stearin and soybean oil was carried out by immobilized lipase Lipozyme TL IM. The reaction curve of batch operation was studied. Results showed that the reaction reached blance in 3 h with 5% lipase at 70℃, temperature of 70~90℃ did no remarkable influence on the reaction rate.

进行了固定化脂肪酶Lipozyme TL IM催化棕榈油硬脂和大豆油酶法酯交换的研究，考察了酶法酯交换分批操作的反应动力学曲线，发现5%的加酶量、70℃下反应，反应3 h内达到平衡，反应温度在70~90℃对反应速度无明显影响。

The tissue of swim bladder membrane provided a perfect micro-environment for glucose oxidase, and adapted to immobilized enzyme.

鱼鳔的生物结构为葡萄糖氧化酶提供了很好的微生物环境，非常有利于酶的固定化。

O fabricate such a hybrid membrane, 3,4,9,10-perylenetetracarboxylic acid-bound thionine moleculeswere initially doped into titania colloids(TiO_2),and then gold nanoparticles and anti-AFP were immobilized onto the composite film in turn.

iO_2成膜能力好，在金电极表面形成大的比表面，有利于增加甲胎蛋白抗体在电极表面的固载量。

The electrochemical characteristics of toluidine blue immobilized within self-assembled silica gel were studied in detail.

实验中，详细探讨了甲苯胺蓝在凝胶组装修饰电极中的电化学行为。

It was shown that urushiol resin immobilized ferric trichloride catalyst was an excellent catalyst.

考察了原料配比、反应时间、催化剂用量等因素对酯化反应的影响。

Isoamyl acetate was synthesized by urushiol resin immobilized ferric trichloride catalyst in this paper.

用漆酚树脂固载化三氯化铁高分子催化剂合成醋酸异戊酯，对催化剂进行了红外光谱表征。

The experimental process was as follows: a set of 5 immobilized copper ion affinity membranes were loaded into a column, uncombined copper ion was removed with 5 mL of sodium phosphate-citric acid buffer (pH 7); the Hb sample volume was 2 mL, and uncombined Hb was eluted with 10 mL of the buffer.

中文内容：摘要：将纤维素滤纸进行碱处理及环氧活化、偶联亚氨基二乙酸、固定化铜离子等处理，并将其装入自制的色谱柱管，制得固定化铜离子亲和膜色谱柱。

After 20 days, the contents of linoleic acid and linolenic acid begin to increase. However, the percentage of stearic acid decreases and the content of oleic acid has no change. So it can be concluded that in immobilized state the fatty acid compositions of plasma membrane have a tendency to shift towards unsaturation and the index of unsaturated fatty acid increases.

同时膜脂肪酸代谢也发生了显著的变化，从第20天后，膜亚油酸和亚麻酸的含量开始增多，而硬脂酸的含量开始下降，油酸的含量没有变化，膜脂肪酸组分趋向不饱和化，脂肪酸的不饱和指数增高。

This text did the research of the part to the physics and chemistry property of terrabacter tunescens and immobilized urease.

本文对肿大地杆菌的理化性质以及脲酶固定化做了部分的研究。

Based on the relationship and the phenomenon model, an improved model of MR damper has been presented in this paper.

摘 要：本文在实验数据的基础上建立了磁流变阻尼器输出力与不同常电流控制量的关系，对现有的 p模型进行了改进。

I don't think our founding fathers were very fuckable.

我认为我们的开国元老没什么吸引力