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On the basis of elastic mechanics theory, the residual microstress expression of matrix around fibers in composites and the stress distribution expression of the matrix around fibers in the composites during tensile process were deduced.

本实验中，纤维体积分数为15%和20%的复合材料强度分别在125℃和81℃时达到了基体的强度，而纤维体积分数为25%的复合材料在室温时强度就超过了基体的。

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC，5％CO。培养箱中培养1－2小时后，弃培养液：lxPBS清洗数次；0.25％胰酶－EOTA 37℃消化5分钟，加入含川％FBS的a－MEM，经小25tim过滤网过滤；IX PBS清洗末过滤细胞，即得到纯化的破骨样细胞，并进行细胞形态学和分于生物学鉴定。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The expression of C-myc is widely high in Rhabdomyosarcoma of female genital system, and the high expression has relationship with tumor＇s histological grade and prognosis.

结论C-myc在横纹肌肉瘤中普遍高表达，并与肿瘤组织学分级和预后相关；在横纹肌肉瘤中，C-myc的表达可能不依赖Ras-MAPK途径调控。

The expression of RACK1 was conserved in various human organs and did not display a robust and stable pattern of rhythmicity in mouse brain. Up and down regulating of Per1 had no significant effects on the RACK1 expression in cell tests.

结果 人下丘脑区域RACK1蛋白与Per1存在相互作用；RACK1在多器官组织保守表达，但其表达未呈现明显节律性；上调及下调Per1表达，RACK1的RNA水平无显著变化。

Twelve (IGF1, P450arom, ESR, FSHR, EGF, PAPP-A, DDP4, NRP2, GnRHR, INHA, PRLR, RBP4) of 14 genes detected for expression differences in ruttish Erhualian and Duroc were found expressing greater in Erhualian's uterus than in Durocs', especially RBP4 (relative quantitation=123.35) and ESR (relative quantitation=10.67) showed highly significant differences in two breeds, which suggested enhancing expression of RBP4 and ESR was favorable to early embryonic survival. These results also suggested high litter size of Erhualianwas the results of integrated effect of many genes

检测了包括NRP2 在内的14 个基因在二花脸和杜洛克中的表达差异，其中有12个基因（IGF1、P450arom、ESR、FSHR、EGF、PAPP-A、DDP4、NRP2、GnRHR、INHA、 PRLR、RBP4）在二花脸子宫的表达量均高于杜洛克子宫的表达量，尤其是 RBP4 和ESR（相对定量结果为分别为123.35 和10.67）表现得最为明显，推测RBP4 和ESR 对二花脸早期胚胎的存活可能有促进作用，由此也可以看出二花脸猪的高产仔性状是受多个基因综合作用的结果。

The result suggests that chewing betel quid containing PBI may activate NF-kB expression that may be one of pathogenesis of OSF. NF-kB expression induced by safrole in fibroblasts may be mediated by ERK activation and COX-2 signal transduction pathway.

实验结果显示，咀嚼内含荖花的槟榔诱发NF-kB，这可能是造成OSF致病原因之一，此外，黄樟素在造纤维母细胞中诱发NF-kB可能是藉由ERK及COX-2传导路径来调控的。

However, IL-18 expression w a s more abundant in the thyroid tissues of HT group than in those of ST grou Th e expression was mainly localized in thyroid follicular cells from HT patients a nd in the mononuclear-macrophages within sarcoid from ST patients .

IL-18在HT组甲状腺组织中的表达明显高于ST组，前者 IL-18表达主要定位于甲状腺滤泡细胞，而后者则主要定位于肉芽肿内单核-巨噬细胞。

The aim of the present study was to study the effect of nicotine on ghrelin and the growth hormone secretagogue receptor expression, the effect of ghrelin on vascular cell adhesion molecule-1(VCAM-1) and interleukin-8 (IL-8) expression in nicotine incubated human umbilical vein endothelial cell,and the possible signaling transduction mechanisms associated with PKC, p38 MAPK and NF-κB.

本实验通过观察ghrelin对尼古丁所致HUVEC VCAM-1及IL-8表达变化过程中PKC、p38 MAPK及NF-κ3的活性变化，并应用PKC、p38 MAPK及NF-κB的特异性抑制剂chelerytherine chloride、SB203580、PDTC，探讨ghrelin阻断尼古丁引起内皮细胞功能紊乱致AS作用的信号转导机制。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。