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The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.

本项目的主要研究结果如下：（1）ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中，质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性；（2）ABA诱导的H2O2产生活化一个46kDa的MAPK，由此而导致编码抗氧化防护酶基因的表达与酶活性的上调；而MAPK的活化也能增强H2O2的产生，从而形成一个正的反馈调节环；（3）水分胁迫也能诱导一46kDa MAPK活化，这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生，同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调；（4）ABA诱导一个依赖于H2O2的NO产生，NO活化一个46kDa的MAPK，从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调；同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。

An expression vector for one isoform of the mbC α1 subunit cDNA was constructed using the human cytomegalovirus promoter to direct expression and this expression vector was used in a novel transfection assay of primary cultures of bovine adrenal chromaffin cells.

为表达具有生物活性的钙离子通道，将mbC的一个异构体的蛋白编码区插入到由人的涎腺病毒启动子控制表达的质粒中。这个表达质粒

Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen Ⅰ and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group, while there was no diference in ACE mRNA expression and activity between the two groups.

结果 APS组血糖、糖化血清蛋白、心肌酶谱和心肌Ang Ⅱ水平较DM组显著下降，胰岛素、C肽、血浆AngⅡ水平和DM组无差异；APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降；APS组chymase mRNA表达和活性均显著低于DM组，ACE基因的表达和活性与DM组无显著性差异。

While levels of insulin. C-peptide and plasma Ang Ⅱ were similar. APS group showed lower levels of chymase mRNA expression and activity than those of DM control group, while there was no diference in ACE mRNA expression and activity between two groups. Compared with DM group, expression level of p-ERK1/2 in APS group was much lower.

结果：APS治疗组GSP、心肌酶谱和心肌AngⅡ水平较对照组显著下降；血胰岛素、C肽、血浆AngⅡ水平与对照组无显著差异；APS组chymase mRNA表达和活性均显著低于对照组，ACE基因的表达和活性与对照组无显著差异；APS组心肌p-ERK1/2含量较对照组明显降低。

The results of histochemical GUS assay showed that no expression of GUSint was observed in Agrobacterium, with transient expression as high as 100% in cotyledonary node of Brassica napus L. after inoculated for three days. It not only verified that the vector was successfully constructed, bur also laid a basis for further increasing the stable transformation efficiency of other Brassica plants, and rapidly clarifying the function and pattern of expression and regulation of a unknown gene.

组织化学染色结果表明，GUSint在农杆菌中没有表达，而在接种3d的油菜中可高效瞬间表达，其中在子叶柄中瞬间表达率高达100%，这一方面证实载体构建成功，另一方面也为进一步优化油菜及其它芸薹属植物转化体系及在油菜中快速研究目的基因的功能和表达调控模式奠定了基础。

objective:our aim is to investigate the relationship of vegf c,vegf d and lymph node metastasis in pancreatic cancer,and to elucidate the role and significance of the cancerous peripheral lymphatic tissue.methods: the expression of vegf c and vegf d was assayed by means of immunohistochemistry in 30 pancreatic carcinomas.results:the positive rates of vegf c、vegf d were 73%(22/30)、57%(17/30) respectively in pancreatic cancer.the expression of vegf c、 vegf d in cancerous invasive edge was significantly higher than that in the center of cancerous tissues.there was no correlation between the expression of vegf c、vegf dand the site,differentiation,histology types.

目的：探讨胰腺癌中vegf c、vegf d蛋白表达与淋巴结转移之间的相关关系，阐明癌周淋巴管增生在胰腺癌淋巴转移中的作用及意义。方法：免疫组化检测30例胰腺癌组织中vegf c、vegf d的表达及其与临床病理、淋巴结转移的关系。结果：胰腺癌vegf c、vegf d蛋白表达阳性率分别为73%(22/30)、57%（17/30），肿瘤周边部位显著高于肿瘤中心部位，其表达与肿瘤的部位、分化程度、组织学类型无关，与肿瘤的tnm分期有关，ⅲ~ⅳ期显著高于ⅰ~ⅱ期。

In the late proliferative and secretive phases, expression of ERα in stromal cells significantly decreased to the level observed in the desquamative phase Differently, no clear expression of ERβ was detected in stromal cells in the late proliferative phase. However, its expression appeared again in the secretive phase.

结果显示： 1ERα和ERβ在恒河猴子宫内膜的上皮细胞、基质细胞中均有表达，其表达水平在正常月经周期中发生周期性变化：上皮细胞中两种受体的表达水平从增殖期开始逐渐升高，到增殖晚期最高；分泌期开始下降，直到月经期的水平。

The SDS-PAGE electrophoresis indicated that all engineered strains containing different expression plasmid could apparently express a 45 kD band specific for daunorubicin C-14 hydroxylase. The expression level of doxA gene was much higher when the doxA gene was under the control of promoter PermE. However, it was found that fd terminator had few effect on the expression of doxA gene.

SDS-PAGE蛋白电泳实验证明，上述五个菌株都能够表达大小约45 kD的柔红霉素C-14羟化酶蛋白，并发现以在PermE启动子控制下的doxA基因表达量相对较高，而终止子对＠中英文摘要 dd基因表达似乎没有影响。

The negative correlation was observed between S100A4 expression and Ecad expression (r=-0.385, P.05) CONCLUSION: The correlated expression of S100A4 and Ecad is closely related to the development of epithelial ovarian carcinoma,which is valuable markers in predicting biological behavior and trend of metastasis in epithelial ovarian carcinoma.

S100A4和Ecad的相关表达与上皮性卵巢癌的发生发展关系密切，是判断上皮性卵巢癌生物学行为、预测侵袭转移的重要指标。

Expression of Caspase-3 and bcl-2 protein In E20 cerebral cortex, the expression of cleaved Caspase-3 in the encephalocoele group are higher than that in control group, and the expression of bcl-2 in the encephalocoele group are lower than that in control group. These findings were consistent with the results of the western blot analysis in E14 Brain.

四、Western-blot检测E14、E20胎鼠大脑皮质中caspase-3、bcl-2蛋白的表达 1、用Western-blot方法检测不同组E20胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平，结果表明对照组、给药无畸形组、脊柱裂组胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平无明显改变，而脑膨出组胎鼠大脑皮质中caspase-3蛋白活化片段表达水平明显增加，bcl-2蛋白表达水平明显减少。

Now she was hungry and angry.She began to smoulder.

现在她又饿又气，她开始流露难以抑制的怒火。

You have placed our iniquities before You, Our secret sins in the light of Your presence.

诗90：8 你将我们的罪孽摆在你面前、将我们的隐恶摆在你面光之中。