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Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Four kinds algorithms are proposed for STPR according to the needs of practice, their propertied are:①the feedfoward network with local feedback has feedback mechanism, it can be applied to identify process with dynamics outputs and static outputs, it can deal with complex process.

2提出四种识别STPR问题的新算法①带有局部反馈环节的前向网络具有局部隐层反馈环节，可同时考虑具有动态输出与静态输出的实际系统的辨识。网络适宜于处理复杂实际过程。

Objective: Parkinsons Disease is a chronic, progressive disease, which is characteristic with tremor, rigidity, bradykinesia and postural problem. The prevalence is 0.1%, which increases to 1-2% in patients with older than 60. PD has resulted in severe burder for family and socialty. In this study, PDQ-39 has been used to investigate the QOL of patient with PD, to identify the damaged dimensions and influencing factors of QOL.

目的帕金森病(Parkinsons Disease，PD)是一种以震颤、肌强直、动作迟缓和姿势反射异常为特征的神经系统进行性变性疾病，患病率为0.1％，在65岁以上人群中达1-2％，给家庭和社会造成严重负担，本研究使用PDQ-39量表对PD患者的生存质量进行评估，确定PD患者生存质量的主要受损维度及主要影响因素，以指导临床医师对PD患者施行合理有效的治疗，并为进一步随访研究作基础。

Spectacular subjects' consumption of the commodity-image transforms them into individuals whose practical needs and choices apparently matter to the order of things, for the narratives of their personal existences assume wondrous qualities that identify them with the symbolico-cultural configurations of their world, while their sensual, idiosyncratic extensions of themselves into the imagistic being of the spectacle, a kingdom of needs and gratified desires, become a means to stamp the world with their expansive individualities, one of whose main qualities, also gifts of the system of appearances, is an expansive tendency that equates life with possibility.

壮观的主体消费的商品形象转换成个人的实际需要和选择，显然问题的顺序外，说明他们的个人存在承担奇妙的素质，确定他们symbolico文化的配置他们的世界，而他们的感官，个体延伸到自己正在意象的奇观，一个王国的需要和满意的欲望，成为一种手段，在世界邮票的个性膨胀，其中一项主要的素质，还馈赠系统的表现，是扩张的倾向，相当于生活的可能性。

The main duty and task have three points:①introduce 、 ingather 、 collect 、 conserve wildness plant 、 local plant 、 breeding resource and genetic material ;②manage seeds resource review 、 introduce 、 exchange 、 inspect 、 propagate 、 update 、 dispense 、 identify 、 appraise 、 utilize 、 finger mark and DNA sequence data ;③The center can supply to decision-making department with information about crop protection and research, to science research and agricultural production with information about resource and to social public with information about protecting biology diversity.

中心内设寒带植物基因资源保存中心、研究与评价中心、黑龙江寒地活体野生资源圃、功能基因库等。其主要职责任务是：①引进、收集、采集、保存植物的野生、地方品种、选育的种质资源和遗传材料；②管理种质考察、引种、交换、监测、繁种、更新、分发、鉴定、评价和利用、指纹图谱和 DNA 序列数据等；③为决策部门提供作物资源保护和研究的信息，为科学研究和农业生产提供优良种质和信息，向社会公众提供保护生物多样性的科普信息。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

The project sorts numbers adopted sort algorithms such as direct insertion sort, shell sort, bubble Sort, quick sort, heap sort, radix sort algorithm respectively with taking random numbers generated by program as key code, Comparing the process of sorting through the consumption of the unit time to objectively reflect the advantages and disadvantages of the various algorithms to sort, identify their problems to the data environment, to enhance efficiency with reasonable sort algorithm to deal with various features of the data to be sort.

本毕业设计通过把生成的随机数作为关键码，分别使用直接插入排序、希尔排序、冒泡排序、快速排序、堆排序、基数排序等排序算法对其排序，通过比较各排序过程所消耗的单位时间来客观体现各种排序算法的优缺点，找出适应它们的问题环境，以合理选择对应的排序算法来高效的处理各种不同特点的待排序数据，提高工作效率。

The entire process very high, from the northeast, Yunnan procurement upper leaf, with eight knife machine cut tobacco, filtered through the expansion of treatment, after a period of storage , and then coupled with being authentic factory smoke oil, import and environmental protection, filter paper, rolled into cigarette sticks taste mellow, neat white ash contraction, together with all packaging machines packaging, simulation rates of up to 95%, non-professional who tobacco companies could not identify.

整个流程极其讲究，从东北，云南采购上等烟叶，用八刀机器切成烟丝，经过膨胀过滤处理后，在经过一段时期的储藏，然后配上正厂地道烟油，进口过滤嘴和环保纸，卷成烟枝味道香醇，烟灰收缩整齐纯白，加上包装一律用机器包装，仿真率可以达到95%以上，非专业烟草公司人士根本无法鉴别。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。