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The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

Abstract］ objective to identify the relationship between serum homocysteine and carotid artery atherosclerosis in patients with cerebral infarction and effects of foliate,methylcobalamin.methods the concentrations of hcy were determined by means of fluorescence polarization immunoassay and carotid arteries was examined with color doppler ultrasound in 165 patients with cerebral infarction.the subjects were divided into 4 groups according to the severity of lesion of carotid artery.the concentrations of hcy were compared in different groups.the patients with hyperhomocysteinemia received foliate and methylcobalamin.results when lesion of carotid artery became severer,serum hcy was higher.the intima medial thicknesses of carotid artery were significantly decreased in patients with hyperhomocysteinemia who received foliate and methylcobalamin for two years.conclusion hyperhomocysteinemia may play an important role in development of carotid artery atherosclerosis.the intervention of foliate and methylcobalamin may reduce carotid artery atherosclerosis.

摘要］ 目的探讨脑梗死患者颈动脉病变与血清同型半胱氨酸水平的关系及叶酸、甲钴胺长期干预对其影响。方法对165例急性脑梗死患者进行血清hcy的测定及颈动脉超声检查，并按动脉硬化的程度分组，比较不同程度颈动脉粥样硬化患者hcy水平，部分高hcy血症患者给予叶酸、甲钴胺干预，随访2年。结果随着颈动脉病变程度的加重，血清hcy浓度呈上升趋势，高hcy血症干预者动脉内中膜厚度明显减小。结论高hcy血症对颈动脉粥样硬化的形成起重要作用，长期给予叶酸、甲钴胺干预可能减轻颈动脉粥样硬化。

One or more matcher components of the system or apparatus compares the received message with stored subscriptions to identify the one or more subscriber programs, generates an alert when an alert condition is satisfied, and compares the generated alert with stored subscriptions to identify one or more subscriber programs subscribing to the alert.

该系统或设备的一个或多个匹配部件将所接收消息与已存预订相比较，以标识一个或多个预订程序，当满足警告条件时生成警告，和将生成的警告与已存预订相比较，以识别预订警告的一个或多个预订程序。

To make a scientific decision, managers may attempt to identify the reparable parts early in the recovery process, to develop a responsible supplier with reactive capacity, or to implement an information system that helps to identify the wear state and number of the used machines that are available for remanufacturing.

为了得到科学的决策，人们试图尽早地掌握拆卸修复零件的确切信息，与有能力的供应商建立良好的关系，或在企业建立管理信息系统来统计分析废旧品的状态和数量等。

Based on the proposed ORPF models, the quadratic optimal models of minimizing the total load curtailment are established to identify power flow unsolvability for AC and AC-DC power systems, and can be further used to identify system voltage instability with the modal analysis method.

算例表明：当离散内点法不存在收敛问题时，启发式混合离散内点法能快速地获取优化解，反之，则要使用启发式混合随机优化算法来保证解的质量，但需要牺牲部分计算速度。

Sales To achieve agreed monthly sales and average transaction value targets for area of responsibility To communicate sales targets and achievement against targets to the sales team on a daily basis To be proactive in identifying selling opportunities and putting in place initiatives that will help achieve and exceed the sales targets Product Knowledge To be able to provide the customer with product information that will enable them to make an informed decision about the item they wish to purchase To be able to communicate confidently the unique selling points and the features and benefits of the product To actively use this product knowledge to provide mix and match ideas for the customer and to up-sell to maximize the sales value Team Leadership To demonstrate and reinforce the Lane Crawford Vision And Values at all times and provide the necessary leadership to gain commitment from retail sales team To identify and execute ways to increase employee's loyalty, satisfaction and pride with the Lane Crawford experience To provide on job training to new hires to ensure they meet the required standards in all aspects of their job within the 3 month probation period To provide on job coaching to ensure that sales associates are able to consistently deliver and exceed the Lane Crawford service standards To train sales associates in product knowledge and mix and match To monitor and review performance of team with the DSM, identifying individuals who are ready for promotion or require performance improvement plans Manage performance of team on a daily basis and take appropriate action with those who fall below standard (in consultation with DSM/HR Department) Housekeeping To ensure daily housekeeping standards are maintained at all times through effective staff scheduling To maintain visual merchandise presentation in line with VM Standards Manual To prevent stock and pilferage by being alert and informing manager/security of potential issues To ensure cashiering function is performed to a high degree of accuracy To ensure stock is continually replenished to ensure a full range of items are available for the customer

顾客服务提供一致、友善和尽责的服务，确保连卡佛的服务标准在任何时间均得到贯彻执行在整个服务过程中积极主动，并提供独特服务，抓住每次机会，为顾客提供超越预期的购物体验向顾客和员工展现了解最新潮流与时尚的热切希望，并积极交流确保每次销售都有售后服务活动跟进，如预定、礼品包装、发送和投递、看管服务确保在零售区域随时有足够的人手提供预期标准的服务，随时提升日常业务水平收集顾客反馈信息并与经理/其他合适人员分享高效并有礼貌地处理和解决顾客投诉，令顾客满意销售达到责任区域内既定的月度销售额和平均交易值目标就销售目标和完成情况和销售团队进行日常沟通积极发现机会并主动创造，帮助达到和超越销售目标产品知识为顾客提供产品信息，使他们在充分了结的基础上对欲购商品做出购买决定能自信地表述产品的独特卖点、特征和优点积极利用产品知识，为顾客提供搭配意见，提升销售业绩，使销售额最大化团队领导随时展示并强调连卡佛理念和价值，展现必要的领导能力，以赢得零售团队的信任发现和执行能提高员工忠诚度、满意度和连卡佛体验自豪感的方法对新进员工的培训进行监督，确保其在3个月的试用期内在各方面均达到工作要求提供工作指导，确保销售助理能一贯执行并超越连卡佛服务标准对销售协理进行产品知识和搭配的培训与DSM一起监督和检查团队的业绩，确定哪些人适合提升，哪些人需要订立业绩改进计划对团队的工作进行日常管理，并对落后员工采取适当的措施内务处理通过有效的员工工作编排，时刻维护日常内务处理标准确保形象商品的展示符合VM标准手册要求保持警惕，就潜在问题通知经理/保安，防止积压和盗窃的发生确保收银职能的精确履行确保货源的持续补充，可随时向顾客提供全系列产品

Special localized cutting communication with the customer; 2.5 If not available notched hole diameter less than 60 mm hole, can be used to be relatively enlarger for the opening; After opening 2.6 backplane approach, starting with the opening negative, positive exposed bit sharp, nated from the positive control (both sides maintain collapse teeth); 3 connected corner of the corner of the 3.1 connection method, identify three Kok code on the middle and lower (50 mm poured from top to bottom. Another try a) 3:00 even in the corner of the counter and cross wooden cabinet on the sideboard; 3.2 corner of the Outer Limits with a shutter-ping; 3.3 In accordance with the drawings corner of the size, will have a fixed angle of the black reinforce the lower corner with 3:00 counters even

特殊局部地方的切割必须与客户沟通； 2.5如割口不大可用圆孔、直径小于60mm孔，可用相对应的扩孔器进行开孔； 2.6后背板开孔方法，先从反面开孔，正面露出钻头尖时，再从正面往里操作； 3 转角条的连接 3.1转角条的连接方法是，找出三个角码，上中下（上下50毫米往里，另居中一个）三点连在与转角柜交叉柜体的侧板上； 3.2转角条的外沿与门板一平； 3.3按照图纸转角条尺寸，将已固定的转角条用黑钉上中下三点与转角柜连

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Construction characteristics - Standard contact and housing dimensions - RAL 7035 grey grip - Cable sleeve for IP44 - Cable gland for IP67 - Plug with key and socket with keyway to ensure perfect coupling - Sockets with spring-locking cover and retaining catch when the plug is inserted, for both IP44 and IP67 - Sockets with locking ring lids and plugs with inclined surfaces and bayonet fitting for IP67 - Fold-back cable clamp for IP67 types - Only factory-preset earth contact position depending on end use - High protection against indirect contacts since the earth contact is the first to established and the last to be broken - Colour-coded to identify the different rated voltages - Perfect interchangeability with EUREKA Series plugs and sockets and with other makers.详情请登入:http://factory.shsap.com/list.asp?id=289

结构特征-触点和外壳标准尺寸-手柄颜色为灰色RAL7035 -堆形过线装置，用于IP44防护等级-填料函，用于IP67防护等级-插头带凸出物，插座带导槽用来保证完好的连接- IP44和IP67 的插座带有弹簧罩，在有插头插入后可以起到止动作用-插座带紧锁罩，插头带有倾斜面用于IP67防护等级的插销式连接-可活动的电缆固定装置用于IP67的防护-在工厂中预设的接地触点位置取决于最终使用-接地触点最先连接，最后短开，所以对防止间接接触具有很高的保护指数-不同的额定电压用不同的颜色来表示，便于识别-与EUREKA系列和其他制造商的插头和插座有极好的互换性

This invention provides a kind of microwave oven that can identify the metal cuisine container. It includes the rotary disk of the cooking room. In the electricity control room there is control circuit. In the control circuit there is main control slug which connects with the current source. Between the power of the control circuit and the main control slug there is alarm circuit and they form a loop with the control circuit. There set the conductive coat and standard riding line on the rotary disk. Through the conductive end set under the rotary disk that the rotary disk's standard riding line connects with, one conductive end connects with the power of the control circuit, and the other connects with the alarm loop and the main control slug.

本发明提供一种具有识别金属料理器皿功能的微波炉，包括有烹调室的旋转盘，电控室中设有控制电路，在控制电路中含有与电源连接的主控芯片，在控制电路的电源与主控芯片之间设置有报警电路并与控制电路形成回路；在旋转盘上设有导电涂层和标准安放线，通过旋转盘上的标准安放线连接设在旋转盘下方的导电端子，其中一导电端子与控制电路中电源相连，另一导电端子与报警电路和主控芯片相连接。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。