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Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Based on a series of signal process and noise source identification techniques, especially the major noise source identification methods, such as expurgation method, sound intensity analysis, spectrum analysis vibro-acoustic correlation analysis and so on, the pass-by noise control of SC7080A and SC6360B are studied in detail. By the roadway and dais experiments, the major noise and radiating parts are identified and some economical controlling methods are made. For the SC7080A, to control the emissing noise from the intake and shell vibration of air cleaner, a intake muffler is installed between the air cleaner and intake valve. Based on the theory of Helmholtz resonator, One three-parallel-connected resonant silencer is designed and trial-manufactured. For the SC6360B, the attenuation performance of exhaust muffler in high frequency region is improved. To reduce the vibration noise of shell part, the structure of oil sump tank, exhaust manifold shield and exhaust manifold shield are analyzed and improved. At the same time, some measures, such as sound absorption, sound insulation, are taken to reduce the noise of engine surface emissivity and a sound shield is designed.

本文首先综述了目前国内外汽车加速噪声控制的现状、主要方法和技术措施，在此基础上，结合SC7080A和SC6350B汽车的车外加速噪声水平及现状，综合运用分别运行法、声强法、频谱分析法、声振相干函数法等现代噪声源识别方法，对SC7080A和SC6360B汽车进行了准确详细的车外加速噪声源识别并提出了经济可行的控制方案，针对SC7080A，主要在空气滤清器和进气阀门之间的进气管道上加装进气消声器，抑制空气滤清器壳体表面和进气口两处的噪声辐射，根据Helmholtz共振腔原理设计了三腔并联旁支型共振消声器，并进行了工程化设计和试制；针对SC6360B，主要改善了排气消声器在高频段的消声性能，对罩壳类零件进行了结构改进，降低了其表面噪声的辐射，并对发动机舱进行了吸声、隔声处理，设计了发动机隔声罩。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

Employees with doctoral, master, engineer or more titles in more than 110 staff, accounting for group one-fourth of the total number, at the same time has set up a dozen experts and scholars in the composition of the External Advisory Board as a group outside the brain; the Group's total registered capital of 50 million; in November 2005 by the Beijing Municipal Committee of Science and Technology identified as high-tech technology enterprises.

员工中具有博士，硕士学位，工程师以上职称的人员一百一十多人，占集团公司总人数四分之一，同时成立了有十余名外聘专家学者组成的顾问委员会作为集团公司的外脑；集团总注册资金5000万；2005年11月被北京市科学技术委员会认定为高新技术企业。

But I am not sure you have correctly identified the positive and negative externalities here.

但我不确定，在这个问题上，你对正外部性或负外部性的判定是否正确。

But I am not sure you have correctly identified the positive negative externalities here.

但我不确定，在这个 www.8 ttt8。 com 问题上，你对正外部性或负外部性的判断是否正确。

Extolment of Zhou in The Book of Songs contains six poems of Mighty Valor Movement. The se poems, realistic and successive in narration, related to a series of historical events in the early Zhou Dynasty, the historical grounds of which can all be identified in time and space.

《诗经周颂》所载大武乐章歌诗六首，它们反映的是周朝初年一系列重大历史事件，在叙事上具有纪实性和连续性，从时间和地域两个方面都可以找到历史根据。

In 3:15 Ezekiel identified himself with the people - he sat among them.

在三章15节，以西结坐在他的同胞中间──换言之，他要与他的同胞认同。

Crown ether was introduced to copolymerize with the monomer of styrene owing to which can associate with silver ions. The silver ions were in-situ reduced to silver nanoparticles by UV light. Then silver nanoparticles were led on the surface of polystyrene latex particles. The vertical precipitation was employed to get the spheres self-assembled into close packed colloidal crystal with a face centered cubic structure identified.

利用冠醚可与银离子复合的特性，制备了聚苯乙烯－冠醚乳胶粒，采用原位还原的方法在乳胶粒表面引入银纳米颗粒，通过垂直沉降，乳胶粒子可自组装成紧密堆积具有面心立方的聚苯乙烯－银胶体晶体。

We called this effect "choice blindness", echoing change blindness, the phenomenon identified by psychologists where a remarkably large number of people fail to spot a major change in their environment.

我们把这种现象称为"选择的盲点"，与"变化的盲点"相对应。后者是心理学家确认到的一个现象，指相当数量的人未能指出他们所在的环境中所发生的某个大的变化。

We are in a real jam.

我们的麻烦大了。

Hey, it's Ahmet from India, that foreign exchange guy.

看，那是印度的阿曼特，国际交换生