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Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

The main causes of the pollution identified result from the irregation of using the polluted water from industries and the endogenic pollution of F and Hg elements is mainly caused by...

造成污染的主要原因是工业三废中的污水长期被利用灌溉，同时查明活动断裂是F、Hg等元素的内生污染源。

With the endophyte strain from fruit and vegetable as tested material, its physiological and biochemical characteristics were analyzed and its morphology and molecular biology were identified.

方法］以来源于果蔬中的内生菌株为试验材料，对其进行生理生化特性分析及形态学和分子生物学鉴定。

According to their individual shapes, they are Gram positive, catenulate rod-shaped bacteria that can produce elliptical endospore in the middle position. These characteristics are similar to Bacillus cereus' described in Bergey's Manual of Systematic Bacteriology. And the colony States, physiological and biochemical characteristics of the two strains also accord with Bacillus cereus', which suggests that they might be identified as Bacillus cereus.

通过菌株的个体形态观察，发现这两株菌是可以在细胞中间产生不膨胀的椭圆形芽孢的革兰氏阳性杆菌，且相互成链，这与伯杰氏手册中对蜡状芽孢杆菌的描述相似，另外对这两株菌的培养特征、生理生化特征的研究，也表明这两株菌符合蜡状芽孢杆菌的特性，故初步鉴定这两株菌为蜡状芽孢杆菌。

First, the overall effect on endothelium should be identified.

第一，需要确定停跳液对内皮的整体影响。

Hence countries around the world have identified the development of new and renewable energy as an integral part of their future energy strategies.

化石能源的应用推动了社会的发展，但资源却在日益耗尽。同时化石能源的无节制使用，造成了严重的环境污染和气候变化问题。

Appropriate clearance of apoptotic cells is an important step of programmed cell death. Although genetic and biochemical studies have identified several genes that regulate the engulfment of cell corpses, how these are degraded after being internalized in engulfing cell remains elusive.

中科院遗传与发育生物学研究所杨崇林课题组以秀丽线虫为模式研究程序性细胞死亡，通过遗传分析、基因表达、蛋白定位等实验手段，发现vps-18基因在凋亡细胞的降解过程中发挥重要作用。

It was identified that the volatile components of mulberry fruit sparkling wine prepared by College of Enology, Northwest Sci-Tech University of Agriculture and Forestry are mainly composed of alcoholic and ester compounds (accounting for 83.72%). The wine had a pleasant aroma characteristic of typical and rich mulberry fruit.

鉴定西北农林科技大学葡萄酒学院研制开发的桑椹汽酒挥发性成分以醇类和酯类化合物为主（占83.72%），酒中香气比较纯正，有典型、浓郁的桑果果香。

The stock being evaluated will consist of cloth in the RM warehouse, in WIP and despatched and enroute to UK so it has to be identified at each stage to ensure we have the full picture.

这次库存清点，包括库存的面料，裁剪后的面料，已发面料和正在运输过程中的面料。因此在每个阶段都要整理明白。

The transient correct expression of oleosin-hirudin fusion gene was identified by SDS/PAGE, western blotting and enterokinase treatment.

介绍oleosin的分类、分布、基本组成、结构与功能、oleosin基因及其表达，讨论oleosin在植物基因工程中的应用。

If you are committed to an education career, then the Master of Arts in Education from Ashford University is for you.

如果你是致力于教育事业，那么，艺术教育硕士学位阿什福德大学是给你的。

objective:to explore the expression of survivin protein and its mrna in rectal cancer and their clinic significance.

目的：研究凋亡抑制因子survivin蛋白及其mrna在直肠癌组织中的表达及其与临床病理关系。