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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

In the present study,using PCR amplification and automatic sequencing of EDA gene coding region,we report five mutations identified in 6 Chinese families with XLHED, including three novel ones:p.M1T,p.L62P and

其中3个点突变(p.M1T、p.L62P和p.G195E)为国际首次报道的新突变。

Autoradiography of aortas demonstrated uptake of the agent into macrophage-rich atheromata identified by Oil Red O staining of lipid deposits.

对主动脉的放射自显影证实该成像剂被通过脂质沉积物的油红O染色鉴定的富含巨噬细胞的动脉粥样硬化部位摄取。

Their morphological and cytological characteristics were observed and identified, comparing with original autotetraploid watermelon.

2001年和2002年在四倍体西瓜品种790010、日み66、91E7和770007中各发现1份类似二倍体西瓜的种子，对这4份突变体进行了形态学和细胞学的观察和鉴定。

In this study, AFLP markers for YrSp gene were identified and SCAR markers were developed using the near-isogenic line YrSp/6 x Avocet S and its susceptible parent Avocet S as materials with the YrSp gene donor parent Spaldings Prolific as control. The main results were given as follows:1. A total of 225 primer combinations were used in AFLP analysis of the materials YrSp/6×Avocet S, Avocet S and Spaldings Prolific.

本研究采用AFLP分析方法，以小麦抗条锈病基因YrSp的供体亲本Spaldings Prolific、近等基因系YrSp/6 x Avocet S、轮回亲本Avocet S及由Avocet S和YrSp/6 x Avocet S杂交制备的F_2代分离群体为材料，开展了小麦抗条锈病基因YrSp的分子标记研究，取得了如下主要结果： 1。

The stable transfected cell Line was selected and identified by RT-PCR awl Western-blotting.

人sTNFR1基因能在NIH3T3细胞系中稳定表达，为今后的研究打下了基础。

A general model supporting the most commonly used scheduling algorithms for multi-core CPU is constructed by a PTL formula S, and the desired property of the system is described by a PTL formula F, then whether the system possesses the property can be identified by proving whether or not S implying P is a theorem based on the axiomatization of PTL.

使用PTL公式建立了支持当前主流进程调度算法的多内核处理器进程调度一般模型S，并将系统期望的性质描述为PTL公式P，在PTL公理系统的基础上，通过证明S蕴含P是否为一个定理来验证系统是否具备该性质。

Vegetative storage proteins in the Melia azedarach L. were identified by using optical microscopy and SDS-PAGE.

以不同季节的苦楝枝条为研究对象，利用光学显微技术和SDS-PAGE技术研究其枝条中贮藏蛋白在休眠期和生长期的分布情况。

Vegetative storage proteinsin the Melia azedarach L.were identified by using optical microscopy and SDS-PAGE.

以不同季节的苦楝枝条为研究对象，利用光学显微技术和SDS-PAGE技术研究其枝条中贮藏蛋白在休眠期和生长期的分布情况。

In the present study, we examined the large-scale gene expression profiles with six sets of matched clinical and experimental fluconazole susceptible and resistant C. albicans strains by microarray analysis. We observed that azole resistances developed in multiple circumstances are all associated with increased expression of CDR mRNA. We also identified some common differential expressed genes and some interesting function-linked gene groups involved in drug-resistance.

利用所构建的基因芯片我们研究了共六对氟康唑敏感和耐药白念珠菌配对菌株的大规模基因表达谱，证实不同环境下产生的白念珠菌耐药性均与多药耐药基因CDR的mRNA表达增加有关，并从中鉴定了一些与药物耐受性相关的共同差异表达基因和一些有趣的功能连锁基因群。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。