查询词典 hybridization

We studied the expression of PTEN gene and its mRNA on a series of 146 cases of primary laryngeal carcinoma patients, 40 cases of precancerous lesion and 26 cases of vocal fold polyp by tissuechip by ,immunohisto- chemistry and in situ hybridization method.

结果：PTEN蛋白在声带息肉中无表达，癌前期病变以及喉癌组织的阳性表达率分别为40.00%、43.15%。

Fig.5 mp53 mRNA in situ hybridization signal in human HXO-Rb44 cells(Fig.a, experimental group,EG;Fig.b, control group,CG).The signal as violet-colored fine granules localized in the cytoplasm,EG weaker than CG,×1 000;Fig.c, mp53 mRNA dot blot signal,EG weaker than CG

图6～8 人HXO-Rb44药品细胞 p16、p21WAF1及Rb 免疫调查组织化学反应性，a为实验组，b为对照组，免疫调查反应细颗粒位于药品细胞核和药品细胞质，EG强于CG，×1 000；c为Rb、p21WAF1及p16蛋白斑点印迹免疫调查反应性，EG强于CG。

Using a three-strand hybridization system, we were able to conjugate oligonucleotides modified Fe2O3 and Au nanoparticles.

经由杂交所形成的复合物(Au/Fe2O3)，在加热至70℃的温度下，会破坏氢键，使复合物(Au/Fe2O3)分开，而回溶於水溶液中。

The study of Comparing with Smear, Culture and PCRreverse Line Hybridization Assay for Detecting Neisseria Gonorrhoeae

核心提示：目的：建立以16S rRNA为靶基因的PCR反向线点杂交技术检测淋病奈瑟菌（Neisseria gonorrhoeae， NG），并与涂片法及培养法比较。

Objective To develop 16S rRNA PCRreverse line blot hybridization assay for detection of N. gonorrhoeae and compare it with smear and culture methods. Methods The DNA from N.

目的：建立以16S rRNA为靶基因的PCR反向线点杂交技术检测淋病奈瑟菌（Neisseria gonorrhoeae， NG），并与涂片法及培养法比较。

It is confirmed that HVA I gene had been inserted into T-DNA by in situ hybridization The effects of variety concentration of Kam .. Cef and Carb on differentiation of 'pinyitiancha'shoot apex were studied.

研究了不同浓度的Kam、Cef、Carb对平邑甜茶茎尖分化率的影响；还研究了预培养时间、不同的农杆菌活化培养基、Ca~浓度、侵染时间、是否使用AgNO~3和PVP、延迟筛选时间对转化频率的影响。

The Western blot and in-situ hybridization were used to study the expressions of VAMP-2 in different tissues of Japanese sea perch.

采用免疫印迹方法和原位杂交技术，研究了VAMP-2在鲈鱼组织中的表达情况，分别对鲈鱼8 种组织中的VAMP-2进行了检测。

Subtractive hybridization was performed by annealing an excess of driver cDNA with each sample of adaptor ligated tester cDNA. The cDNAs were heat denatured and incubated in 68℃ for 8 hours.

杂交产物经过LA Taq DNA聚合酶补平末端后，以试剂盒提供的PCR Primer 1作为第一次PCR的引物，以Nested Primer 1和Nested Primer 2R作为第二次PCR的引物，通过抑制性PCR富集大量在Tester中高表达的差异表达基因的cDNA。

The expression of S100A4 gene in samples of cancerous tissues and corresponding non-cancerous tissues from 61 colon cancer patients was detected by using in situ hybridization.

它们与很多不同的生理功能相关，比如细胞的增殖、细胞外信号的传导、细胞间的黏附和运动，以及癌细胞的转移等。

Results The recombinant plasmid pSNAV2.0-TK was verified byPCR and restricton endonuclease. The coat protein of AAV was detectedby SDS-PAGE method, the purity of rAAV2/HSV-TK was＞98% byHPLC method and the titre of rAAV2/HSV-TK was 1×10~(12)v.g./ml byDNA dot blot hybridization.

结果：重组质粒pSNAV2.0-TK PCR扩增的片段大小和酶切鉴定所切下的片段与预计结果一致；SDS-PAGE法检测到三条特征性的AAV外壳蛋白条带，HPLC法检测rAAV2／HSV-TK的纯度＞98％；用DNA斑点杂交方法检测rAAV2／HSV-TK的滴度为1×10~(12)v.g。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。