查询词典 hybridization

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体，故可在体外转录cRNA时同时得到意义（Sp6为启动基因）和反意义（T7为启动基因）探针，实验结果表明，cRNA探针标记率高，杂合物稳定，故所获杂交反应背景较为满意。

College of Life Science, Wuhan University, Wuhan 430072On the basis of optimization of in situ RNA hybridization technique, Westudied the temporal and spatial expression of calmodulin and phosphorylasemRNA and its relation with fertilization and embryogenesis in rice as well as antherdevelopment in tabacco. We also constructed cDNA libraries of proembryos anddifferentiating young embryos in rice using microscopic isolation and RT-PCRtechniques.

本文在优化RNA原位杂交技术的基础上，研究了钙调素mRNA和磷酸化酶mRNA时空表达及其与水稻受精和胚胎发生以及烟草花药发育的关系；利用显微分离技术和RT-PCR技术建立了水稻原胚和分化幼胚cDNA文库。

Five pairs of primers were designed for PCR according to five fragments of the putative virulent genes acquired by suppression subtractive hybridization between virulent strain HA9801 and avirulent strain 12 of Streptococcus suis type 2 （ SS2 ）. The five fragments were transcriptional regulator (15# and 154# fragments), amino acid permease (210# fragments), ABC transporter (9# fragments) and surface anchored protein (187# fragments).

通过猪链球菌 2 型（ Streptococcus suis type 2， SS2 ）毒力菌株 HA9801 与无毒力菌株 12#之间的抑制性差减杂交实验，获得了可能与 SS2 菌株毒力相关的 5 个基因片段，分别是转录调节子（ 15#，和 154#片段），氨基酸通透酶（ 210#片段）， ABC 转运子（ 9#片段）及表面锚定蛋白（ 187#片段）。

Low levels of transcripts were also detected in other organs as well as mutant ovules by RT-PCR and Northern hybridization.

进一步的分析表明GhABC1基因在纤维细胞中的表达量很高，在子叶、叶片、茎等组织中低水平表达，在根中则检测不到其转录本。

Methods: 60 oviducts and placental villi from tubal pregnant women were tested for the presence of HSV DNA by in situ hybridization. Additionally, 20 normal oviducts were used as control.

方法应用非放射性核酸分子原位杂交技术检测60例异位妊娠患者输卵管及绒毛组织HSV DNA的存在情况，并与正常输卵管比较。

Up to now, a series of hybridization offsprings were obtained.

测序结果表明，该条带长362bp，在子代与父本中的一致性为100％。

The results show that the diffraction patterns for WS2 single phase were observed; the pronounced quantum size effect exists in S-W-S nano clusters, and it enhances the hybridization of different electronic shell obits and formed a closed hollow spherical structure without any dangling bond. In the lubricating process, such a system can maintain its chemical stability and decrease energy dissipation.

分析结果表明：XRD图样显示为WS2相单相；在S-W-S纳米簇团中存在显著的量子尺寸效应，该效应强化了硫原子电子壳层间的轨道杂化，使纳米级的WS2润滑晶体形成了1个没有悬键的、化学性能稳定的中空球体，在润滑过程中，这种结构可使体系保持较强的化学稳定性，能耗降低。

Using immunocytochemistry and in situ hybridization, we studied definitive tissue specific localization of aromatase in the nervous system, wheel organ, Hatschek's pit and gonads at young and adult female and male amphioxus at different stages of gonadal development.

本文用免疫细胞化学和原位杂交技术，首次发现芳香化酶活性组织特异性定位在幼年和性腺发育不同时期雌、雄文昌鱼神经系统、轮器、哈氏窝和性腺中。

Male sterility of wheat inducing by Chemical hybridization agents is one of the main ways to produce hybrid wheat seed.

应用化学杂交剂诱导雄性不育是小麦杂种优势利用的重要途径之一。

The fluorescence in situ hybridization technique has established itself as a powerful improvement over conventional cytogenetic techniques and is now a well-accepted procedure for chromosome analysis and aneuploidy screening.

相对于传统的细胞遗传学方法，原位杂交技术具有很大的改进，已成为一种公认的染色体分析和非整倍体筛选技术。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。