查询词典 hybridization

Methods pna conjugated fish (fluorescence in situ hybridization)-fitc was performed on paraffin sections from melanoma,melanoma metastases and common naevi, respectively. telomere mean maximum diameter, area and number per nuclear area were calculated by image analysis on fluorescence images elaborated through confocal laser scanning microscope and fluorescence microscope.

应用肽核苷酸探针标记荧光原位杂交—荧光素异硫氰酸法检测黑色素瘤、转移黑色素瘤及黑色素痣石蜡切片中的端粒，以荧光显微镜、激光共聚焦显微镜生成图像，并详细记录细胞核平均面积、端粒数目、端粒面积。

Using in situ hybridization, we found that ribozyme transcripts were predominantly located in the nucleus of transgenic potatoes, consistent with the hypothesis that ribozymes were more efficient against nuclearly replicative viroid.

原位杂交结果表明核酶转录产物主要分布在转基因马铃薯细胞的细胞核中。这一结果支持我们提出的核酶对在核内复制的类病毒更为有效的假说。

Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.

利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide，oligo)探针和PCR探针点样于玻片，并以植物18S rRNA作为内参照制成基因芯片。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

N-methyl-N＇-nitro-N-nitrosoguanidine was used as a known teratogen to induce cleft palate in ICR mice and a suppression subtractive hybridization technique was applied to identify differentially expressed genes which related to cleft palate in ICR mice.

方法以甲基亚硝基胍为受试物诱导腭裂动物模型，采用抑制消减杂交技术从全基因组水平上对MNNG致腭裂相关差异表达基因进行筛选。

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

In this study, we used RNA whole mount in situ hybridization to analyze the expression pattern of the dGATA-II gene in the embryonic stage, and found it expresses in the brain、central nervous system、midgut and posterior spiracles at different stages.

由於过去研究，使dGATA-II基因结构已被清楚了解，本论文承续前者，对dGATA-II转录因子在果蝇胚胎时期，促使细胞发育达到特定形态的功能进行探讨。

Using the cDNAs from primary spermatocytes and round spermatids, the cDNA subtractive libraries of these cells were constructed by the application of the technology of suppression subtractive hybridization.

我们得到了这个蛋白质的晶体并且对它进行了初步的晶体学分析。人类FcaRI(CD89)分子是黏膜和血清中含量极其丰富的IgA抗体的特异性受体。

Methods The serotyping, hybridization and RAPD typing methods were used. Seven strains of GBS were detected in 2 pairs of mother-baby, which included 2 strains from the 2 mothers and 5 strains from their babies with early and late-onset infections. Eighty-eight strains of GBS were detected in vaginas of pregnant womens.

采用血清学、核酸杂交和PARD方法对从2例诊断为早发型GBS肺炎和晚发型GBS脑膜炎患儿体内分离的5株GBS和其母阴道分离的2株GBS菌株作母婴配对分析，对88株孕妇生殖道携带GBS进行RAPD分型研究。

Progenies of distant hybridization between double low rapeseed NJ5280 and Descurainia Sophia were selfed in three consecutive generations. After individual plant selection and quality analysis, 13 yellow-seeded plants were obtained from 430 F3 plants. Among them, there were 4 plants having more than 45% oil, less than 0.5% erucic acid and less than 30μmol/g glucosinolate.

对双低甘蓝型油菜(NJ5280)与播娘蒿远缘杂种自交后代进行选择和品质鉴定，在F3群体中得到13份黄籽材料，其中有4份黄籽双低高油油菜新种质，其品质符合含油量大于45%、硫苷低于30μmol/g、芥酸小于0.5%的双低高油标准。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。