查询词典 hybridization

New PlatinumBright nucleic acid labeling kits offer a proprietary ULS labeling molecule bound to a choice of fluorescent dyes or haptens, for use in fluorescence in situ hybridization applications.

新型PlatinumBright核酸标记试剂盒具有知识产权的ULS标记分子，可以连接荧光染料或者半抗原，用于荧光原位杂交实验。

Vulgaris and Beta M14 BAC clone 16-M11 and 26-L15 are florescence specific expression BAC of Beta M14, both BAC clone 16-M11 and 26-L15 were in situ hybridization to monosomic addition lines(2n=2x=18+1) M14 chromosomes, they were located on the long arm of corollinae chromosome 9. the dominant factor of BAC clone 16-M11 and 26-L15 appears to hemizygous, because no equivalent region has been found in sexual Beta.

选用这两个BAC克隆作为探针，对M14甜菜有丝分裂中期染色体组进行荧光原位杂交，结果发现，BAC克隆16-M11和26-L15定位于M14甜菜附加的一条白花甜菜9号染色体上，有性栽培甜菜染色体上没有出现信号，两个BAC克隆在M14甜菜中呈半合子状态，遗传行为象单价显性基因，是白花甜菜9号染色体特异的。

Expression of hbFGF in the RPE cells was determined by in situ hybridization and immunohistochemical met hods.

于荧光显微镜下观察GFP的表达情况，用原位杂交和免疫组织化学染色法检测bFGF在RPE细胞的表达情况。

While hybridization happens and "is a natural process," according to Kays, it's also not mating per usual.

尽管杂交是经常发生和一种自然行为，但在狼群当中是很罕见的。

"We are finding repeatedly that hybridization is more common than we used to think," lead author Roland Kays told Discovery News.

证据指出它们两者的杂交现像比我们想像中更为普遍。

The expression of VEGF, bFGF and BMP-2 were detected in femoral head through making use of immunohistochemistry and in-situ hybridization technique.Results The organization structure of experimental group was disorganized cracked and there were a great number of empty lacunas in bone trabecula.

所有标本取材后经固定、脱钙后，制成切片，光镜及电镜观察其病理变化及骨修复情况，并以免疫组化和原位杂交技术对其VEGF蛋白、bFGF与BMP-2mRNA的表达情况进行检测。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针，并将合成的特异探针固定在硝酸纤维素膜上，与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应，通过观察杂交信号判断存在何种细菌感染，同时研究该方法的敏感性和特异性。2。

On the base of the above-mentioned results, this paper further investigated the local micro-actuating behaviors induced by the volume change of PPy doped with ssDNA on the microdot electrode within the microchannel. It was found that, in 80μm channel the volume change of PPy film doped with ssDNA probe could enhanced the effect of DNA hybridization.

在上述结果的提示下，论文进一步在微通道内初步考察了在ssDNA掺杂修饰的PPy微点上因其电化学氧化还原所致的体积变化率而造成的局部微致动行为，发现在80μm微通道内掺杂了ssDNA探针的PPy薄膜的体积变化足以引起随后DNA杂交效果的变化。

Methods PNA conjugated FISH (Fluorescence in situ hybridization)-FITC was performed on paraffin sections from melanoma,melanoma metastases and common naevi, respectively.

应用肽核苷酸探针标记荧光原位杂交—荧光素异硫氰酸法检测黑色素瘤、转移黑色素瘤及黑色素痣石蜡切片中的端粒，以荧光显微镜、激光共聚焦显微镜生成图像，并详细记录细胞核平均面积、端粒数目、端粒面积。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。