查询词典 hybridization

Methods In situ hybridization and immunohistochemistry were used respectively to detect mRNAs and proteins in 47 cases of esophageal squamous cell carcinoma and their correspondent para-cancerous mucosae, distal cuts.

目的：研究EGR-1 、c-fos 、cyclin D1 3种基因mRNA及其相应蛋白在人食管癌癌变过程中的表达水平及其相关性，并探讨其与食管癌的发生、发展及生物学行为的关系。

Methods The expression of CD44 mRNA was examined in cancerous tissues and adjacent noncancerous mucosae from 26 patients with colorectal cancer and in colorectal polyps from 7 patients by reverse transcription-polymerase chain reaction and Southern blot hybridization.

应用逆转录、多聚合酶链反应和Southern杂交技术，检测26例大肠癌患者癌组织、正常大肠粘膜组织和7例大肠息肉CD44基因mRNA表达。

Methods:Twenty-one gastric carcinoma tissues and correponding non-cancer mucosae were examined by in situ hybridization with digoxigenin-labeled nm23-H1cDNA probes.

此后，在多种动物模型或人类某些实体癌中发现nm23-H1基因表达水平与肿瘤转移呈负相关［2～5］，但少数作者的实验结果表明，nm23-H1基因与转移无关［6，7］。nm23-H1基因在胃癌中表达仅见少数报道［7，8］，结果不一，本组实验应用地高辛标记cDNA探针和免疫组化方法检测石蜡包埋组织中nm23-H1基因及其表达产物nm23/NDPK在胃癌中的表达，探讨nm23-H1基因表达与胃癌的临床病理关系。

Methods Rats were infected with MP for 9 times during a period of 24 weeks by ultrasonic nebulizing inhalation to establish animal models of chronic pulmonary MP infection. In situ hybridization was performed with bFGF cDNA probe and results were quantitatively analysed to measure the changes of bFGF mRNA expression in lung tissues.

将8只大鼠于24周内反复9次经超声雾化吸入肺炎支原体以复制其慢性肺部感染模型，另以4只为对照，随后用bFGF cDNA核酸探针行核酸分子原位杂交及定量图像分析，观察肺组织bFGF mRNA表达的变化。

Epstein\|Barr virus and Human papilloma virus in sinonasal papillomas: An in situ hybridization and polymerase chain reaction study［J］.

鼻腔鼻窦内翻性乳头状瘤中人类乳头状瘤病毒和EB病毒DNA表达的研究［J］。

Maternal serum HBV DNA level was quantitated by molecular hybridization techniques.

孕妇外周血HBV DNA定量分析采用分子杂交法。

It is a good way to analyze the expression level of the gene of retinas with the technique of in situ hybridization.

原位杂交技术为研究视网膜基因表达状况的一种良好方法。

It is a good way to analyze the expression level of the gene of retinas with the technique of in situ hybridization.

关于光化学损伤状况下，视网膜中SOD基因mRNA表达水平的研究，目前国内外尚未见报道。

The experiment has built a method to sexing the hybridization of chicken and quail in early stage of embryo development.

推测杂交种胚胎死亡的时间集中在69-72h，这一时间段胚胎的死亡，是导致整个孵化过程胚胎死亡率升高的主要原因。

This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.

文章系统地介绍了最新发展的microRNA定量检测方法，详细阐述了基于探针杂交技术的Northern blotting法、微阵列芯片法、纳米金标记法、桥连同位素标记法，以及基于扩增技术的定量PCR检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等，并对这些方法的优缺点进行了分析比较。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。