查询词典 hybridization

The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization.

免疫动物：实验组18 只豚鼠每只用200 μg/0.2 ml 粗制膜迷路抗原加等量完全弗氏佐剂制成的乳化液行首次免疫。

Methods A model of autoimmune inner ear disease was established by systemically immunizing the guinea pig with the homologous crude inner ear antigen. The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization.

利用同种异体内耳抗原免疫豚鼠，以建立自身免疫性内耳病（autoimmune inner ear disease， AIED动物模型，并应用免疫组织化学及原位杂位技术，研究热休克蛋白（heat shock protein， hsp70在正常对照组及 AIED 模型动物实验组耳蜗中的表达情况。

During the past 200 years, cottons have evolved hybridization to become the industrial cottons we know today.

在过去的200年中，经过杂交，棉花演变成今天的工业用棉。

During the past 200 years , cottons have evolved hybridization to become the industrial cottons we konw today.

在过去的200年中，经过杂交，棉花演变成今天的工业用棉。

Moreover, because the mulberry is a cross-pollinated plant, it is hard to judge the relations among the species accurately because of the hard up for phylogenetic information in transition types formed in intra-species and inter-species hybridization, therefore, there is no satisfied classification method for Morns L..

因此，利用分子生物学技术研究桑属植物的系统与进化关系，有助于解决我国桑属植物分类中所存在的问题，对于桑树种质资源的分析、鉴定和利用、桑树的遗传育种都具有十分重要的意义。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

Membrane eDNA microarray containing 3'-EST of 512 known rice genes was used for monitoring the expression profiles of geminating rice seedling, and 313 genes generated reliable hybridization signals.

使用含512个已知水稻基因3'表达序列标签的cDNA微阵列检测了萌发期水稻幼苗的基因表达谱。313个基因产生了可靠的杂交信号。

The results are as followings: the CHA SQ-1 has the best effect of male sterility among three kinds Chemical Hybridization Agent, and has the ability to increase 1000 kernel weight. The CHA GENESIS, especially BAU9403 reduces the 1000 kernel weight. The SQ-1 and GENESIS have no significant differences with CK on the traits of geminating potential, germinating rate and volume weight, but BAU9403 has significant differences with CK. SQ-1 is a promising CHA in this experiment. It will be used in commercial seed production instead of GENESIS.

结果表明，SQ-1在3种化学杂交剂中的杀雄效果最好，并对杂交种种子的千粒重有提高作用；GENESIS和BAU9403不同程度对杂交种种子千粒重有降低作用，其中BAU9403影响更大；SQ-1和GENESIS对杂交种发芽率、发芽势和容重的影响与对照不喷药无显著差异，但BAU9403与对照有显著差异。

So researchers use a known strand of DNA as a target, and then compare how much of an unidentified strand hybridizes in comparison to the amount of hybridization seen with the known complementary strand.

比较起来，杂交纤维的总量似乎多于已知的互补纤维总数。

Methods Transgenic mice and the intercrossed progeny between the founder mice of human Cathepsin-K gene and wild ICR were detected by Southern blot hybridization with DIG labeling probe.

组织蛋白酶K是一种溶酶体含有的半胱氨酸蛋白酶，它有选择性的在破骨细胞引起的骨吸收中起特殊的作用[1，2 ] 。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。