查询词典 hybridization

The Tongfuxingshen liquid proctoclysis can promote the patients in unconscious state to conscious state, and improve the degree of coma and the scores of nervous functional defects,To observe the expression of HSP70, HO-1mRNA and the Cerebral vessels permeability, encephaledema in rat brain after experimental haemorrhagic stroke. Hemorrhagic stroke Sprague Dawley rat model was made by injecting collagenase type Ⅶ and hepathrom stereotaxically, Rats were randomly divided into fake operation group, model group and enema group, take orally group, in three time point (3 days, 5 days, 7 days) we observed the change of cerebral vessels permeability, encephaledema and HSP70 immunohistochemistry, HO-1mRNA hybridization in situ.

结果表明，通腑醒神液直肠滴注较灌胃起效快，能尽快促使昏迷患者神志清醒，意识清醒时间平均为4.94天，而灌胃组为6.9天，经统计学处理有极显著性差异；明显改善患者的昏迷程度，第8天治疗组Glasgow-Pittsburgh昏迷评分值平均为29.39分，对照组为26.60分，经统计学处理有极显著性差异；治疗后14天临床疗效比较，通腑醒神液直肠滴注组总显效为54.84%，总有效率为80.65%，而灌胃组总显效为43.33%，总有效率为66.67%，经统计学处理无差异性，可能为样本量过少有关，尚需进一步增加样本量评估。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能医疗增强病毒在宿主体内的适应性和生存，又有可能医疗使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能增强病毒在宿主体内的适应性和生存，又有可能使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

Besides, we firstly confirmed at the molecular level that P450arom may play an important role in the natural sex change of protandrous fish and revealed that up-regulation of the transcript levels of P450arom during the course of natural sex change of black porgy is not regulated via FTZ-F1.;sturgeon sex related and sex linkage genes by candidate gene approach and suppression subtractive hybridization technique and expression patterns of these genes were also studied.

首次直接在分子水平证明芳香化酶基因可能参与了雌雄同体鱼类性别逆转的调控，而黑鲷性别逆转期卵巢中芳香化酶的表达量上调不是由FTZ-F1基因调控的；同时主要利用候选基因法和抑制差减杂交搜寻史氏鲟性别决定或性别特异性基因，并研究其表达情况。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针，进行原位杂文，研究POMCmRNA在大鼠卵巢中的分布，实验表明，POMCmRNA主要分布于间期，妊娠和假孕大鼠卵巢黄体中，间质细胞也可见少量POMCmRNA，而卵泡颗粒细胞中POMCmRNA极少。

Methods Rattus hearts in embryonic days 12, 15, 19 were included in this study. The mRNA level of HCK gene expression was detected by the methods of RT-PCR and in situ hybridization, and the results were analyzed by the statistical methods.

方法取大鼠胚胎12、15、19d心脏，采用RT-PCR技术检测心脏组织中HCK基因mRNA的表达丰度，并应用原位杂交技术对HCK基因表达进行定位分析。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

Rubrospinal neuronal cross-ectional area was measured to determine if atrophy had been reversed, and in situ hybridization for GAP-43 and T1 tubulin was performed to determine if there genes, which are important for axonal regeneration, were up-egulated.

另外，还测定了GAP-43 和T1微管蛋白的原位杂交是否上调。它们对于轴性再生是非常重要的。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。