查询词典 hybridization

Site was detected near the centromere of metacentric chromosome, while the other homologous one had no hybridization signal.

在石蒜染色体上发现9个杂交位点，在1号长臂端部、5号近端部区域和6号长臂近着丝粒区域各有3个位点。

METHODS: 8-Bromo-cAMP and sodium nitroprusside were added into the media to raise the concentrations of cAMP and cGMP of cultured cardiomyocytes, in situ hybridization and immunohistochemistry methods were used to identify the mRNA and protein expression of bFGF. The amount of bFGF mRNA and protein expression were detected by computor imaging analysis system MIAS exe.

分离培养大鼠心肌细胞，加入8-溴-cAMP和硝普钠，升高细胞内cAMP、cGMP浓度，用原位杂交及免疫细胞化学的方法观察细胞内bFGF的表达，图象分析检测bFGF表达量的变化。

Micrococcal nuclease hypersensitive DNA fragments were either cloned directly, or mapped onto the genome by hybridization with oligo-nucleotide probes on a 244K Agilent array-CGH chip. The array-CGH mapping was confirmed for nuclease sensitivity by PCR after MNase digestion and by Southern blot analysis.

我们利用小球菌核酸酶超敏感的去氧核醣核酸片段直接选殖或是将其作为寡核苷酸探针於安捷伦244k寡核苷酸阵列-比较基因体杂合晶片上，进行基因体定位，并利用聚合酶链索反应和南方墨点法侦测其核酸酶敏感程度，以确认结果之可靠性。

The journal publishes papers embracing all aspects of histochemistry and microtechnic in all areas of the biological sciences from botany to cell biology to medicine, including in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, image analysis, cytogenetics and automation or computerization of investigative procedures.

杂志刊载了内容涉及生物科学中从植物学到细胞生物学到医学所有方向的组织化学和显微技术所有领域的论文，包括原位杂交，细胞化学探针，放射自显影，光和电子显微镜，组织培养，图象分析，细胞遗传学和自动或计算机研究方法。

The journal publishes papers embracing all aspects of histochemistry and microtechnic in all areas of the biological sciences from botany to cell biology to medicine, including in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, image analysis, cytogenetics and automation or computerization of investigative procedures.

杂志刊载了内容涉及生物科学中从植物学到细胞生物学到医学所有方向的组织化学和显微技术所有领域的论文，包括原位杂交，细胞化学探针，放？？？？？？？自显影，光和电子显微镜，组织培养，图象分析，细胞遗传学和自动或计算机研究方法。

The results showed that there was hybridization signal on metaphase chromosome and interphase nucleus of human,but no signal on metaphase chromosome and interphase nucleus of Microtus mandarinus.According to the results,we discussed the homologue between pY3.4 in Y chromosome of humanbeing and DNA of Microtus mandarinus and FISH result of Microtus mandarinus.

结果显示，人的中期染色体上和间期细胞核中发现了杂交信号，棕色田鼠的未发现杂交信号，由此讨论了棕色田鼠DNA与人Y染色体pY3.4的同源性和棕色田鼠的FISH结果，以便进一步研究棕色田鼠Y染色体。

The results of Fiber-FISH test in maize indicated that the hybridization signal was the extended moniliform long chain, the length of telomere DNA in each chromosome of maize was 7~103 μm and the copy numbers of the repeated sequences of telomere among each chromosome had significant differences (15~230 kb).

玉米的Fiber-FISH试验结果表明，杂交信号为伸展的念珠状长链，玉米各条染色体端粒DNA的长度为7~103μm，各染色体端粒重复序列的拷贝数存在显著差异（为15~230 kb）。

Interspecies hybridization and backcrossing is a means to transfer desirable genes from one species to another in breeding programs of higher plants. Monosomic alien addition lines can be produced via addition of single chromosome of an alien donor species to the entire chromosome complement of the recipient species.

在高等植物中，以种间杂交和回交把有益基因从一个物种转移到另一个物种为目的育种项目中，单个外源染色体常常被附加到含有受体细胞完整一套染色体中，形成异源单体附加系。

Hybridization of cDNA probe and gene chip was carried out, gene chip was scanned with Scan Array3000, and results were analyzed with ImaGene 3. 0. Result: 1 Toluidine blue staining of semithin section showed: compared with control group, the boundary of tunica intima, tunica media and tunica adventitia was not distinct, tunica intima and internal elastic membrane was thickened and multilaminar.

将标记了荧光素的cDNA与细胞周期相关基因表达谱芯片杂交，扫描并分析杂交结果。cy5/cy3 ratio值在0.5～2.O之外且一对Double基因表达趋势一致的基因为表达有差异基因，ratio值＞2.O表明DM组该基因上调，ratio值＜0.5表明DM组该基因下调。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。