查询词典 hybridization

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis.

体外分离培养Ast，利用划伤的方法，建立Ast对损伤反应的实验模型，通过形态学观察、RT-PCR、免疫细胞化学、原位杂交及图像分析等方法，研究体外Ast对损伤的反应及规律。

These shoots were examined by PCR,PCR-Southem hybridization and DNA dot blotting.Results indicated that the geneencoding A1aB1b subunit of glycinin has been actually introduced into mulberry.

用PCR、PCR-Southem及DNA斑点杂交等方法对这些Km抗性苗进行了鉴定，结果证实大豆球蛋白基因确已被导入桑树体内。

By using genome in situ hybridization on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum × G.

然而，仅依据形态学和普通的核型图像，还不能确定该异源四倍体棉种中比克氏棉G染色体组在核型中的表现。

A Study on Distant Hybridization of Gossypium hirsutum L.

甘肃省农业科学院经济作物研究所，甘肃兰州 730070

Morphology of sexual reproduction abortion in'High Noon', a distant hybridization cultivar of tree peonies(P.delavayi var. lutea×P. suffruticasa), was systematically investigated with light microscopy and cross. The results indicated that:(1)The stamens matured earlier than the gynoecia and pollens dispersed at the early stage of anthesis but the rate of normal 2-celled pollens were merely 2.41%.

采用光镜和杂交等方法对牡丹远缘杂交一代品种'正午'有性生殖败育进行系统形态学观察发现：(1)雄蕊较雌蕊先成熟，初花期即散粉，正常2-细胞花粉仅占2·41%，且花粉在多种培养液中、在自身和其它两种柱头上均不萌发；柱头在谢花期大量分泌粘液，进入最佳授粉状态；(2)同一胚珠中多个大孢子母细胞减数分裂高度不同步，胚囊发育与受精延迟，部分胚乳游离核在细胞化时期分裂异常；(3)自花及品种内异花授粉不结实，天然授粉结实率为0·053‰~0·130‰。

Wu Jie does not idle, he integrates the 12 abalone farmers in capital and technology advantages, the Czech Republic was set up in Fuzhou Feng Seafood Development Co., Ltd., and Xiamen University, College of Oceanic and environmental cooperation, the introduction of abalone in Japan and the West's original abalone Haliotis hybridization temperature to produce good, strong disease-resistant varieties of the new "building on the 1st Czech Republic," and "Feng Jie One", so that the survival rate of abalone 90%.

吴捷也没闲着，他整合了12家鲍鱼养殖户的资金技术优势，成立了福州捷丰海珍品开发有限公司，并与厦门大学海洋与环境学院合作，引进日本西氏鲍与原有的皱纹鲍杂交，培育出适温性好、抗病力强的新品种&厦捷一号&和&捷丰一号&，使鲍鱼的成活率达90％以上。

Fluorescence in situ hybridization and immunofluorescence assay revealed that the DNA sequences associated with the heterochromatic knobs are highly divergent and heavily methylated compared with the sequences in the corresponding X chromosomal domains.

而通过NDA的分子原位杂交，专家在番木瓜的雄性区域发现了该区域有4个特异的异染色质疖，免疫荧光分析则揭示出这个区域与X染色体的相应区域发生了较大的分化，具有高度的甲基化。

The whole-mount in situ hybridization was used to detect the distribution of lin-28 and other three heterochronic genes in T. spiralis.

因此，我们利用原位杂交技术研究了lin-28及与其相关的3个异时性基因在旋毛虫中的分布情况。

One histiocytic sarcoma showed t(14;18) by quantitative-polymerase chain reaction, which was confirmed by fluorescence in situ hybridization analysis showing IGH/BCL2 fusions in neoplastic histiocytes.

定量性聚合酶链反应显示1例组织细胞肉瘤发生了t(14；18)，此结果也得到荧光原位杂交分析的证实，即肿瘤性的组织细胞发生了IGH/BCL2基因的融合。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。