查询词典 hybridization

The barley chromosome in wheat was identified by genomic in situ hybridization in which biotin labelled total genomic DNA of barley betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring as blocking DNA. A series of wheat materials were tested as follows; two disomic alien substitution and monosomic alien addition lines, 2n=43; two monosomic alien substitution lines.

用生物素(Biotin-16-dUTP)标记的大麦Betzes基因组DNA作探针，以普通小麦中国春总DNA作封阻进行基因组原位杂交(Genome in situ hybridiz ation，简称GISH)，从13株小麦-大麦杂交后代中鉴定出2个含有3条大麦B etzes2H染色体的材料(2n=43)；2个2H单体异代换系(2n=42)；7个2H二体异代换系(2n=42)。

Report gene and in situ hybridization indicate the expression of OsPTF1 in leaf, lateral root and root phloem except for epidermic cells in root.

通过报道基因和RNA原位杂交分析表明，OsPTF1在叶片各组织中均表达，在侧根和根的韧皮部区域表达，但在表皮细胞中不表达。

Fluorescence in situ hybridization technique was used to study the distribution patterns of 5S rDNA and 18S rDNA on chromosmoes of six species of Epinephelus.

这种同源染色体异形的现象在石斑鱼属中属首次发现。4、银染结果表明，在6种石斑鱼种内和种间，NORs数目和分布模式具有多态性。

Epinephelus; Grouper; Chromosome; FISH fluorescence in situ hybridization

石斑鱼；染色体；荧光原位杂交

Four obvious up-regulated ESTs of SSADH were obtained in the drought stress Erianthus arundinaceus througth cDNA microarray hybridization.

通过消减文库技术结合cDNA芯片技术筛选的EST中含有4个推测为基因SSADH的EST，其在水分胁迫处理的斑茅中均明显上调表达。

Basing on our early stage research work about collection and description of sugarcane germplasm and the aided project of Yunnan Natural Science Fund (2001 C0015Q), the wild species Erianthus fulvus, which has good characters, cold-resistant, drought-resistant, higher Brix and less chromosome number, was utilized for the first time through intergeneric hybridization with sugarcane cultivar. Its F_1 hybrids were appraised with some new techniques and methods for germplasm enhancement.

本研究在课题组考察采集和保育鉴定甘蔗野生种质资源的前期工作基础上，依托云南省自然科学基金资助项目(2001C0015Q)，首次利用具有特异优良性状(抗旱、耐寒、锤度高、染色体数目少)的蔗茅野生种与甘蔗栽培种或品种进行属间远缘杂交，扩大甘蔗遗传多样性；采用新技术、新方法对蔗茅杂种F_1代材料进行早期鉴定评价研究，筛选具有育种价值的新种质，为进一步选育生态适应性广、抗逆性强、丰产高糖的甘蔗新品种奠定物质基础。

In this research, we attempted to construct an SSH (suppression subtractive hybridization) library using the leaves of wild H. villosa (Gh21) inoculated by Erysiphe graminis and its susceptible EMS-induced mutant M14S with no inoculation.We screened 218 positive clones in the SSH library, and gained 60 ESTs after sequencing.

本研究应用抑制性消减杂交技术，以野生型簇毛麦(Gh21)及其感病突变体(M14S)为材料，建立白粉菌诱导的叶片的SSH文库，获得218个阳性克隆，对其中部分克隆测序，获得60个EST序列。

In this report, subtractive hybridization coupled with polymerase chain reaction was used to identify genes related to erythroid terminal differentiation from FVA cells cultured with EPO for 36 hours.

因此我们选用了FVA细胞为材料，用减除杂交与PCR相结合的方法，克隆小鼠红系细胞终末分化阶段的红细胞分化相关基因，得到了一个红系细胞的中晚幼阶段差异表达的cDNA片段并对其进行了初步的功能研究。

Objective To investigate estrogen and andro gen on the regulation of gene expression of insulin-like growth factorⅠmRNA in prostatic tissues. Methods IGF-ⅠmRNA was quantitat ively ass essed in BPH and SD rat prostatic tissues using dot blot hybridization technique .

目的 探讨雌、雄激素对列腺中胰岛素样生长因子Ⅰ基因表达的影响方法采用斑点杂交结合图像分析技术，观察药物去势和未药物去势BPH患者前列腺组织以及正常组、去势组、外源性雄激素组、雌激素组鼠前列腺组织中IGF-ⅠmRNA表达情况。

Methods IGF-ⅠmRNA was quantitat ively ass essed in BPH and SD rat prostatic tissues using dot blot hybridization technique .

采用斑点杂交结合图像分析技术，观察药物去势和未药物去势BPH患者前列腺组织以及正常组、去势组、外源性雄激素组、雌激素组大鼠前列腺组织中IGF

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。