查询词典 hybridization

［Purpose］ To explore the application for clinical assessment of HER2 gene expression status by chromogenic in situ hybridization and to compare the results with those obtained by immunohistochemistry.

［目的］探讨色素原位杂交在检测乳腺癌患者组织中HER2基因状态的临床应用，比较CISH与免疫组化检测组织HER2状态的差异性。

The positive rate of cloacal swab from non immunized chickens and SPF chickens experimentioly infected with AIV were 10%(1/10) by dot hybridization on the third day p.i..

该探针具有较好的特异性和敏感性，为从分子水平探讨 AIV的发病机理、临床早期快速诊断提供了新的研究手段。

The basic diploid number of chromosomes in the family Cobitidae was deemed to 2n=50. The C . sinensis with 2n=40 may originate from the ancestor of the subfamily Cobitidae. The C sinensis with 2n=90 which reserved all of 40 original chromosomes maybe result from heterologous hybridization among some fishes of the subfamily Cobitidae. The C . sinensis with 2n=76 is probably a branch of hybrid offspring.

鳅科鱼类染色体的祖先二倍数是2n=50，2n=40的中华花鳅可能是由类似于泥鳅的2n=50的鳅亚科祖先经罗伯逊易位2n=50→48→40转变而来的。2n=90的中华花鳅保留了初始的40条染色体，它可能是由2n=40的中华花鳅与同为鳅亚科的其它鱼类杂交后演化而来的。2n=76的中华花鳅并非异源三倍体，它可能是杂交后演化的一个分支。

The knob signals were very strong and clear only for the hybridization of Tripsacum dactyloides (2n=72) with genus Zea instead of Coix lacryma-jobi (2n=20) with them.

这在一定的程度上反映，供试的三个属中，玉蜀黍属和摩擦禾属亲缘关系更为相近，而与慧该属的关系相对地远些。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

White spot syndrome virus ; copepoda ; resting eggs ; PCR-dot-blot hybridization

对虾白斑综合症病毒；桡足类；休眠卵； PCR斑点杂交法

In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV. The results showed that WSSV positive cases were detected in copepoda from egg to adult, and the positive copepoda appeared earlier than cultural shrimp in ponds.

1。本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵，以及由这些休眠卵孵化出的幼体携带病毒情况，发现桡足类由卵到成体各阶段中均有阳性个体，而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。

In the present study,in order to explore the roles of estrogen and progestin in epilepsy and elucidate their mechanisms of action, we utilized animal seizure models induced by intracerebroventricular coriaria lactone and intraperitoneal bemegride, studied the effects of estrogen and progestin on central nervous system functions in behavior,electrophysiological,cellular,molecular and gene levels by means of neuroelectrophysiology, flow cytometry, high performance liquid chromatography, immunocytochemical and in situ hybridization techniques.

为了探索雌、孕激素在癫痫发病中的作用，阐明其作用机制，本工作分别以马桑内酯侧脑室注射致痫、贝美格（Be腹腔注射致痫大鼠为实验对象，采用神经贫血电生理、流式细胞美白免疫荧光、高效液相色谱、美白免疫细胞化学、原位杂交早泻技术，从整体、行为、细胞、分子以及基因水平研究发现了雌、孕激素对大鼠中枢神经贫血系统功能的影响。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。