查询词典 hybridization

A simple and inexpensive BAW DNA biosensor has been proposed by immobilizing DNA on an Ag-plated surface. The surface modification and the process of dissociation and hybridization of DNA have also been studied.

成功地将DNA修饰于压电晶体Ag晶片表面，构成DNA生物传感器，并对其表面修饰过程和DNA杂交过程进行了分析研究。

By the method of in situ hybridization, the distribution of mRNA of RSG6 was detected. The result showed that the transcript activity began at the late unicellular stage microspore, increased at the bicellular pollen, and was most vigorous at the two sperm cells in mature pollen.

用地高辛RNA标记试剂盒，采用体外转录方法标记RSG6基因的cDNA序列中长度为258bp的一段作为反义RNA探针，运用RNA原位杂交技术，探讨了RSG6基因的mRNA在水稻根、茎、叶以及花药发育各阶段组织中的分布情况。

Key words: Boophilus microplus;suppression subtractive hybridization;expressed sequence tag;sequence analysis

微小牛蜱；抑制消减杂交；表达序列标签；序列分析

Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046, China）Abstract: With cDNA from salivary gland of semi-engorged adult female Boophilus microplus as tester and cDNA from starved adult female B.microplus as driver,a subtractive cDNA library of salivary gland of female B.microplus was constructed using suppression subtractive hybridization and T/A cloning.

以我国微小牛蜱半饱血雌性成蜱唾液腺cDNA为检测子Tester，饥饿雌性成蜱cDNA为驱动子，采用抑制消减杂交技术和T/A克隆技术构建了微小牛蜱半饱血雌性成蜱唾液腺消减cDNA文库。

In caecum、colon、rectum,very strong GnRHR mRNA hybridization signal was also detected in mucosa epithelium and glandular epithelium.

盲肠、结肠和直肠的粘膜上皮细胞、肠腺上皮细胞也能检测到GnRH受体mRNA杂交信号。

In this study,an experimental modelwas developed and established with freshwater crayfish Cambarus clarkii to prolif-erate White Spot Syndrome Virusin vivo.The success and reliability ofthis model was confirmed by transmission electron microscopy,dot-blotand hybridization in situ etc.

本研究在阐明WSSV对常见淡水甲壳纲动物的侵染活性的同时，成功地建立了WSSV淡水克氏螯虾体内增殖模型，并通过电镜技术、核酸探针斑点杂交与原位杂交等手段证实所建立的增殖模型的可靠性。

Ferrocenecarboxylic-ssdeoxyribonucleic acid probe was made by chemically bounding FCA to terminal 5'-amino of oligonucleotide in the presence of a water-soluble carbodiimide. The hybridization reaction was conducted by immersing the immobilized-ssDNA electrode in FCA-ssDNA probe solution.

在偶联活化剂碳二亚胺的存在下，通过羧基二茂铁上的羧基与DNA分子上的氨基共价键合，将FCA标记在氨基修饰的寡聚核苷酸片段上，制备成FCA-ssDNA探针。

Abtract Objective To study the level of the expression of PTTG mRNA in the cholecystic tissues of gallbladder adena?鄄carcinoma, chronic cholecystitis and gallstone. Methods In situ hybridization was used to detect PTTG mRNA on the routinely paraffin?

目的 研究胆囊腺癌、慢性胆囊炎和胆囊结石病例的胆囊组织中垂体肿瘤转化基因（pituitary tumor transforming gene， PTTG）mRNA表达水平及其临床病理意义。

The presence of the t(15;19) chromosomal translocation in the chondroid component was confirmed by fluorescence in situ hybridization analysis and immunohistochemical staining, indicating mesenchymal transdifferentation of the tumor.

荧光原位杂交实验和免疫组化染色证实其软骨样成分发生了t(15；19)染色体易位，由此表明该例肿瘤发生了间叶组织转分化。

Chromogenic In situ hybridization is used to detect specific nucleic acid sequences in tissue, cell, or chromosome aberrations.

显色原位杂交法可用于对组织/细胞或染色体畸变中的特异核酸序列进行检测。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。