查询词典 hybridization

Some species of sturgeons are polypolids. The hybridization is common even between genera Acipenser and Huso. The parents of different species with the same chromosome numbers will produce reproductive offsprings, and thus, genetic introgression among species would easily happen in the nature conditions.

鲟具有多倍体特徵，其中鲟属及鳇属杂交的情形非常普遍，染色体倍数相同的亲代杂交产生的子代具有繁殖力，因此在自然情况下或人为移殖时很容易造成基因渗入现象。

Methods miR-145 expression was detected in 91 cases of breast carcinoma and 38 cases of benign breast lesions including 21 cases of fibroadenoma and 17 cases of adenosis on tissue microarray sections by in situ hybridization.

目的 研究一种微小RNA(miR-145)在人乳腺癌及乳腺良性病变组织中的表达，分析miR-145的表达与乳腺癌临床病理特征的关系及其对乳腺癌发生发展的意义。

Methods The model of middle cerebral artery occlusion was set up in mature rats using the technology of in situ hybridization and radioimmunoassay to dynamically observed and to quantitatively analyse the expression levels of CRH messenger ribonucleic acid in the limbic systen and the levels of adrenocorticotropic hormone in plasma.

利用鼠大脑中动脉阻塞模型，应用原位杂交和放射免疫分析法，监测对照组和缺血组鼠脑颞叶、海马和下丘脑的CRH mRNA转录水平和血浆ACTH的活性变化。

In situ hybridization and GUS staining analyses revealed the up-regulation of LEAFY , AP1, PISTILLATA and AGAMOUS in pAP1::IPT4 transgenic plants.

原位杂交和GUS组织染色结果发现，在pAP1：：IPT4转基因植株中，花分生组织特征决定基因LEAFY与花器官特征决定基因AP1、PISTILLATA和AGAMOUS的表达量均有不同程度的提高。

Then sacrificed the rats in every group to get cornu dorsale medullae spinalis and to make frozen section.The changes of orphanin receptor mRNA which was related with algesia in cornu dorsale medullae spinalis were observed by hybridization in situ.

各组动物处死后，取脊髓背角组织，冰冻切片，采用原位杂交的方法，观察各组脊髓背角Ⅰ～Ⅵ层内与痛觉相关孤啡肽受体mRNA的变化。

The HE and the VG stain were pathologically examined and the expression of TGF-β1 and TNF-α mRNA in the lung tissues were analyzed by nucleic acid hybridization in situ. Results: YQHX1,2,3 obviously alleviated rats alveolitis and fibrosis.

结果：模型组肺泡炎和肺纤维化程度较重，且其胶原纤维沉积较多，TGF-β1、TNF-α的表达增多；而中药治疗组肺泡炎和纤维化程度较轻，胶原沉积明显减少，TGF-β1、TNF-α表达减少。

The detectable group, biotin-ε-aminocaproic acid N-hydroxysuccinimide ester, is attached to the remaining amino group. A series of multiamine compouds, i. e. ethylendiamine, 1, 6-hexandediamine, triethylenetetramine, tetraethylenepentamine, and synthetic polyarylamide are used inlabelling, it is found that linker arms and steric hindrance effect the detection sensitivity. The probes labeled by 1, 6-hexandediamine and triethylenetetramine can detect 0.1-0.5pg target DNA in dot blot hybridization, and their sensitivities are better than the others.

本文详细考察了乙二胺、己二胺、二乙烯三胺、三乙烯四胺、四乙烯五胺及合成的聚乙烯胺化合物对λDNA探针的标记和检测的影响，发现胺化物链长和空间位阻影响探针的杂交和检测，用己二胺和三乙烯四胺标记的生物素探针在杂交24小时、显色8小时后检测灵敏度达0.1-0.5pg，超过其它胺化物标记的探针。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

A new Antheraea pernyi variety Hulunbei'er 1 in bivoltine area in which breeding one generation for a year has been bred by the methods of pedigree separation and hybridization.

采用系统分离和杂交育种方法，育成了适合二化一放蚕区放养的柞蚕四元杂交新品种呼伦贝尔一号。

The apomictic monosomic addition line of Beta corolliflora, designated as M14(VV+IC, 2n=18+1), was obtained from remote hybridization of B. vulgaris L.

本实验室的郭德栋教授通过远缘杂交方法获得了甜菜无融合生殖单体附加系M14品系(VV+1C，2n=18+1)，以下简称甜菜M14品系。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。