查询词典 hybridization

With this model, we constructed an apoptotic cDNA library by subtractive hybridization which was based on PCR.

在此基础上，应用基于PCR技术的消减杂交技术，建立与凋亡相关的前列腺癌细胞DU-145的差异cDNA文库。

Subtractive hybridization is a method to be used to isolate sequences presenting in one mRNA population but not in others.

减除杂交是一种用于克隆差异表达基因的方法。

In virtue of the technique of suppression subtractive hybridization, we had successfully constructed a subtractive library of D.

本研究利用新近发展的抑制性差减杂交技术成功构建了珙桐幼嫩苞片和叶片差减文库。

The improved PCR-based subtractive hybridization might be efficient for cloning of differentially expressed genes in target cells.

结论这种基于PCR技术的改良消减杂交方法对于差异表达基因的克隆是十分有效的。

Fan YS,Davis LM,Shows TB.Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosome.Proc Nat1 Acad Sci USA,1990,87∶6223-6227.5 Cherif D,Julier C,Delattre O,et al.Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy:Application to regional mapping of human chromosome 11. Proc Nat1 Acad Sci USA,1990,87∶6639-6643.6 Harai M,Suto Y,Kanoh M.

我们曾经介绍过一种荧光R显带的方法［10］，在此基础上建立了一种在显带染色体上直接观察杂交信号的新的定位方法：在细胞培养时，同时加入BUdR和Hoechst33258，PI染色后即呈现清晰的荧光R带带型，用彩色和全色黑白胶卷照相均可得到满意的结果，尤其适用于国内具有一般条件的实验室。

A rapid and reliable method for the identification of classical swine fever virus genotypes based on oligonucleotide microarray hybridization has been developed.

本研究应用寡核苷酸基因杂交技术建立了一种快速可靠的检测猪瘟病毒的方法。

In this research , by using of genomic in situ hybridization , random amplified polymorphism DNA technology and three specific SCAR molecular markers, the author analyzed wheat hybrid generations carrying the desired genes that were resistant to take-all, BYDV and/or powdery mildew, and developed some alien chromosome addition and translocation lines that were important in breeding practice, and some specific molecular markers that could be applied in marker-assisted selection.

本研究运用GISH、RAPD和SCAR分析方法，对抗全蚀病、黄矮病和兼抗黄矮病白粉病的小麦杂交后代材料进行分析，获得了一些对育种实践有重要意义的易位系、异附加系材料和几个可用于辅助育种的特异性分子标记。

In hybridization in situ sections, there were no iNOS positive cells in FO group, iNOS positive cells were found in other 3 groups in mucous layer , tela submucosa and vascular wall.

原位杂交DAB染色：假手术组均未见到iNOS阳性细胞，其他组胃粘膜全层均可见到阳性细胞，包括粘膜层及粘膜下层的细胞、血管壁。

The objective of this study was to compare the effect of distant hybridization between a new teosinte Germplasm named 'LUMU No.2' and 11 temperate maize inbred lines on the mode of dependency and field in long sunlight condition.

采用保护地和大田种植两种栽培模式，以"鲁牧2号"大刍草新种质为父本，11个温带玉米自交系为母本，对其在长日照条件下的远缘杂交效果进行了比较研究。

Hybridization of diploid and tetraploid is an effective way to breed seedless triploid citrus.

利用二倍体与同源四倍体杂交是培育无核三倍体柑橘的一种有效方法。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。