查询词典 hybridization

Methods: In this study, the ontogeney of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization vsing a digoxigeninlabeled RNA probe.

采用地高辛标记的RNA探针，用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶C产生细胞的个体发育。

METHODS: During permanent tooth root formation of dog MSX1 mRNA was detected by in situ hybridization.

采用原位杂交技术，对犬恒牙牙根不同发育时期MSX1mRNA的表达进行观察。

Methods:The expression of bmp3,bmp4 and bmp7 mRNA at different stages of the development of permanent tooth roots was examined by in situ hybridization in 3 dogs aged 12-18 weeks.

采用原位杂交染色技术，观测犬恒牙牙根发育各阶段标本中bmp3、bmp4、bmp7mRNA的定位表达。

At the same time, through the atom ratio of chlorine to phosphor, the hybridization efficiency of the DNA probes anchored on silver film was calculated to be 90.81%, which was largely higher than those probes affixed on conventional glass substrate (30.63%).

与此同时，通过氯原子与磷原子的个数比得到了银膜DNA芯片上杂交效率为90.81%，高于常规玻璃载体基因芯片的杂交效率（30.63%），说明银膜DNA芯片能够实现碱基序列正错配的分析，自组装化学镀银膜是一种应用前景较好的基因芯片载体材料。

Through photoemission experiments,the 3d- 4s hybridization and very high density of 3d states can be demonstrated clearly.

由于杂化使得3d带的空穴参与了导电过程，而高的态密度使其具有极高的有效质量。

Keywords Plastrum testudinis;Mesenchymal stem cells;Bone morphogenetic protein 4;In situ hybridization;Rat

龟板； 骨髓间充质干细胞；骨形态发生蛋白4；原位杂交；大鼠

Whole chromosome painting probe ; first polar body ; fluorescence in situ hybridization ; oocyte

全染色体涂抹探针；第一极体；荧光原位杂交；卵母细胞

Recently, it is proved that SP is also involved in pain modulation, acupuncture analgesia and immunoin- hibition. However, the role of CGRP in them is unclear Therefore, the dy- namic changes of CGRP-, SP-like immunoreactivity and expression ofα- CGRP,β-CGRP mRNAs in dorsal root ganglion and spinal cord in the arthritic rats were observed by using immunohistochemistry and in situ hybridization techniques combined with behavioral tests, respectively. Subsequently, the effects of this peoptide on the develoment of polyarthritis were investigated by intrthecal injections of CGRP and its antagonist in the rats. In addtion, GAL is a novel neuropeptide which coexists with CGRP and SP, but it may play a different role in the primary sensory system. Thus, we also observed the changes of GAL and GAL mRNA in DRG neurons and the spinal cord. The results were as follows

为此本文拟在大鼠佐剂性关节炎模型上，结合行为学测定、用免疫组织化学、原位核酸分子杂交技术观察脊髓背角及DRG中CGRP、SP、α-CGRP mRNA、β-CGRPmRNA的动态变化及电针的影响，此外，用鞘内和外周注射CGRP的工具药探讨了CGRP在佐剂性关节炎发生发展中的可能作用；甘丙肽（Galanin，GAL）也是新近发现的一个神经肽，并与CGRP和SP共存于DRG及初级传入末梢中，在神经损伤模型中脊髓CGRP、SP减少，GAL则明显增加，显示GAL与CGRP和SP的作用可能不同，因此在佐剂性关节炎模型上也同时观察了GAL和GAL mRNA的变化，实验结果如下

Within this context, microseparation and in vitro culture of early zygotic embryos from polyembryony Citrus is very important because it makes possible the use of immature hybrid embryos and accelerates the sexual hybridization breeding in Citrus.

本研究初步建立了多胚性柑桔早期合子胚的显微分离、离体培养及合子苗的鉴定体系，成功显微分离了柑桔早期合子胚并通过离体培养再生植株，具体结果如下： 1、多胚性柑桔早期合子胚显微分离的最适时期为授粉后50—55d。

In conventional sexual hybridization of citrus, nucellar polyembryony, pollen/ovule sterility, genetic heterozygosity, wild sexual incompatibility of most citrus cultivars make it very time-consuming and low efficiency to improve fruit characters.

但是，由于柑橘类植物中存在的珠心胚现象、一些品种中的雌雄性败育、童期过长、远缘有性杂交不亲和等特点使通过传统的有性杂交方式进行品种改良进程缓慢，效率不高。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。