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The vasa gene expression pattern during oogenesis of zebrafish was examined using in situ hybridization and fluorescent quantitative RT-PCR.

原位杂交结果表明：在卵母细胞生长、发育的过程中，第Ⅱ期卵母细胞的细胞质中vasa mRNA大量表达。

And it also showed that the inheritance of the growth-rate and pathogenicity to cotton seedlings of XC-6 could not be inherited steadily in zoospore progenies and oospore progeny, but the colony morphology and homothallic character of XC-6 could inherited steadily in both asexual and sexual progenies, which suggested that the inheritance and variation of the above characters might be related to long-selfing and hybridization in species.

同时，XC-6生长速率、对棉苗的致病力、菌落形态及同宗配合遗传和变异特性研究表明，其生长速率和对棉苗的致病力性状在单游动孢子后代和自交后代均不能稳定遗传，但菌落形态和同宗配合特性在单游动孢子后代和自交后代均能稳定遗传，提示上述性状在单游动孢子后代和自交后代的遗传和变异特性可能与菌株长期自交和种内杂交有关。

Preferential oxidation of Ti on NiTi alloy surface has been observed and was consistent with the experimental result and the free energy of formation A G?(298K) of NiO, TiO, TiO2. Mulliken population and the partial density of state analysis showed that the interaction between Ti and O atoms was mainly donated by O 2p and Ti 4s orbital and O 2p and Ti 3d, 4s, 4p hybridization orbital. The electron structure in NiTi bulk material was not altered during 62 molecule adsorption process and the surface passivation oxide formation.

进一步对钛与氧之间相互作用本质的研究表明，O 2p轨道和Ti 4s轨道以及O 2p轨道和Ti 3d 4s 4p杂化轨道间的相互作用是NiTi合金表面氧分子吸附的驱动力，在此过程中，NiTi合金的体相结构没有发生变化，从而保证了其一系列力学性能和形状记忆特性在表面氧化后不发生明显的变化，使表面改性的工作可以安全、可靠的完成而不影响其植入人体后发挥正常的生理功能。

Mulliken population and the partial density of state analysis showed that the interaction between Ti and O atoms was mainly donated by O 2p and Ti 4s orbital and O 2p and Ti 3d, 4s, 4p hybridization orbital. The electron structure in NiTi bulk material was not altered during O〓 molecule adsorption process and the surface passivation oxide formation. Thus the mechanical properties and shape memory effect would not change after NiTi alloy surface oxidation. Stable microstructure demonstrates reliability of implant material.

进一步对钛与氧之间相互作用本质的研究表明，O 2p轨道和Ti 4s轨道以及O 2p轨道和Ti 3d 4s 4p杂化轨道间的相互作用是NiTi合金表面氧分子吸附的驱动力，在此过程中，NiTi合金的体相结构没有发生变化，从而保证了其一系列力学性能和形状记忆特性在表面氧化后不发生明显的变化，使表面改性的工作可以安全、可靠的完成而不影响其植入人体后发挥正常的生理功能。

Forty adult female rats were divided randomly into 4 groups:① Pseudo-operation control group: no ovariotomy, no common carotid artery occlusion;② Cerebral ischemia reperfusion group: with CCAO only, no OE;③ Ovariotomized control group: with OE and CCAO, no estradiol;④ Estradiol supplement group: with OE, CCAO and estradiol supplemented (30 d after ovariotomy, estradiol was supplemented every day for 14 d). All the rats were sacrificed after 12h of reperfusion injury, 10% of brain tissue was homogenized and 10% alue was determined by UV spectrophotometer, NO content was detected, and frozen sections and NOSmRNA in-situ hybridization were performed.

取SD大鼠40只随机分为4组：①假手术对照组：不切除卵巢，不夹闭颈总动脉；②脑缺血再灌注组：作局灶性脑缺血再灌注手术，不切除卵巢；③去卵巢对照组：切除卵巢，作局灶性脑缺血再灌注手术，不补充雌激素；④补充雌激素组：切除卵巢，作局灶性脑缺血再灌注手术，补充雌激素（切除双侧卵巢30d开始补充雌激素，连续2周）。

Methods: To establish the rat model of spleen-deficiency by the methods of purgation with bitter and cold nature dyugs, omproper diet and overstrain. Immunohistochemistr and in situ hybridization were used to tetect and C-fos and C-JUNmRNA expression in the hypothalamus ventral kernel, the hippocampi CA1 area and forehead cortex.

采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型，免疫组化法、原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层C-fos水平与C-JUNmRNA表达变化及归脾汤的治疗作用。

Methods and Results— In 23 paired myocardial samples, cardiomyocyte ploidy was investigated by DNA image cytometry, flow cytometry, and in situ hybridization.

方法和结果-在23个配对心肌样本中，通过DNA图像定量分析，流式细胞仪和原位杂交来探测心肌细胞整倍体。

METHODS: The transcriptional levels of BMP5 in the control and treated embryonic palatal shelves were detected by in situ hybridization with advancing development.

采用原位杂交技术观察在不同发育时期，正常胎鼠和腭裂胎鼠腭突中BMP5 mRNA的表达情况。

Crosssing apomictic rice and C4 crop,such as Sorgum,Trapsicum and Panicum maximumespecially by somatic hybridization and gene engineering technique to create new varieties with characteristics of apomixis and high photosynthetic efficiency.

利用无融合生殖水稻与无融合生殖高光效的C4作物，如高粱、摩擦禾、木黍等杂交，特别是体细胞杂交和基因工程技术，创造具有无融合生殖特性高光效作物新品种。

This is because of the relative paucity of hybridization in the fungi as compared with green plants.

这是因为真菌中的杂交同绿色植物比较起来相对贫乏的原故。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。