查询词典 hybridization

Results There are 13 positive cases in 30 in situ Hybridization tissues of NPC, the p16 gene mRNA positive rate is 43.3%, and the p16 gene mRNA are positive in all of 20 cases of chronic Nasopharyngitis.

结果 30例NPC标本原位杂交阳性13例，p16 mRNA阳性表达为43.3%，3个高发家系的NPC标本无阳性表达，20例对照全部有p16 mRNA阳性表达。

Methods: The expression of EOMES gene, ETS-1 gene and MEF (myeloid Elf-1 like factor) gene in 25 cases of N-NK/T-L and 25 control cases including 11 cases of B cell lymphoma (BCL, 6 cases of T cell lymphoma, 3 cases of normal spleen, 5 cases of chronic nasopharyngitis were detected by tissue microarray and in situ hybridization.

通过对EOMES，ETS-1和MEF基因进行克隆、探针制备和组织芯片制备，对25例N-NK/T-L以及同部位11例B细胞淋巴瘤、6例T细胞淋巴瘤、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。

Methods The expression level of UPA/UPAR mRNA were detected by using in stiu hybridization in the tissues of the nasopharyngnal carcinoma and the chronic nasopharyngitis.

方法用mRNA—DNA原位杂交技术检测鼻咽癌组织和炎症组织UPA和UPAR的基因表达水平。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示，石蜡切片的鼻咽癌癌巢内，可见53BP2的强阳性表达信号，信号呈紫色颗粒状分布于胞核，核仁清晰可见；而在相同条件下，在慢性鼻咽炎组织和癌旁上皮的结缔组织，也可见弱表达信号，如淋巴细胞和平滑肌细胞；在增生的鳞状上皮细胞的核内，可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上，均无表达信号。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

By RT-PCR and in situ hybridization, we found Pmr1 was expresses from early neurula (st.15) in the ventral part of embryo, then located in the anterior ventral blood island which is the primitive myeloid cells generated region.

Pmr1是从非洲爪蟾肝组织提取出的mRNA核酸内切酶，参与雌激素诱导的mRNA降解过程。但是Pmr1在胚胎发育中的分布和作用尚不明了。

The best result of hybridization is produced in nitrocellulose membranes.

进口硝酸纤维素膜的灵敏度最好，本底信号低。

It has opened a new way for the diagnosis and therapy of tumours, and also estab- lished the foundation for the carcinogenic mechanism research. In order to study the altered molecular events during laryngeal car- cinogenesis and elucidat the role of oncogenes in laryngeal carci- noma, we examined the Ha-ras and c-myc gene mutation and amplification by the polymerase chain reaction and molecu- lar hybridization technigues respectivly.

癌基因的研究是当前肿瘤领域内的重大课题，正在基础理论和临床医学中进行广泛和深入的研究，癌基因的发现为肿瘤的诊断和治疗开辟了新的途径，同时也为人类开始从分子水平来回答细胞癌变的本质问题，最终认识癌变机制奠定了理论基础。

With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外，我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况，结果发现28例干燥综合征病人中16例为阳性，其中腺泡组织破坏严重者，EB病毒DNA的含量亦高，并且EB病毒主要分布于大量浸润的淋巴细胞中，其次位于腺泡上皮细胞内，少量见于腺泡腔中，以上结果提示，在干燥综合征病人EB病毒处于活跃的复制状态，干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

Methods:Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors.

采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中p27蛋白和mRNA的表达状况。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。