查询词典 hybridization

Only one complete corollinae chromosome was detected in Ml4, and was determined corollinae chromosome 9. We have used in situ hybridization of genomic DNA to discrimination the parental chromosomes in Beta Ml 4, suppression of cross-hybridization by blocking DNA was not necessary indicating that the investigated Beta genome contain sufficient species-species DNA enabling the unequivocal determination of genomic composition of the hybrids.In our study , the results of BAC -FISH combined with the analysis of GISH in Beta M14,indicating that present of the corollinae chromosome 9 alone was sufficient to confer apospory in Beta M14 upon celluar and molecular..

BAC克隆16-M11和26-L15携带M14花期特异表达的基因；在M14甜菜对应的有性栽培甜菜染色体上没有出现信号，呈半合子状态；包含的单或低拷贝DNA序列在野生白花甜菜进化过程是共线的；两个BAC克隆部分同源；位于末端重组抑制区，所有这些不同寻常的特性都暗示着：BAC克隆16-M11和26-L15和无融合生殖特性相关，可作为无融合生殖

The influence of different ph of the fixative, different fixation time, different pre hybridization treatment, and different pre hybridization and hybridization solutions were compared.

然后比较不同ph值(7.5~9.5)的固定剂，不同的固定时间，不同的杂交前预处理，以及不同预杂交及杂交液对杂交信号的影响。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

Chicken and quail intergeneric hybridization is a classic distant hybridization in fowl. In fowl science, chicken and quail belong to Aves, Galliformes, Phasiamidae, Gallus and Coturnix, and this hybridization combination has become the example of researching the incompatibility of different germ cell and sterile of distant hybridization.

鸡与鹌鹑杂交是属间杂交，其杂交种胚胎早期出现大量的死亡，并且杂交种出壳以前雌性胚胎全部死亡，这使鸡与鹌鹑杂交成为研究异种生殖细胞不亲和性和杂交种不育的典型。

In addition, the authors suggest:① The research of firs hybridization should be carried out on a large scale in China;② In hybridization, the phylogeny of Abies must be considered, and we should put emphasis on the intro-section hybridization;③ The research on the strobili phenology of firs can ensure the hybridization succeed;④ The research is also important for the hybridization to study the genetic diversity of Abies;⑤ We can use the biotechnology and the knowledge of modern molecular biology to overcome the difficulties of breeding.

作者对我国开展这项研究提出了几点看法：①应大力开展我国冷杉树种间，以及与国外冷杉树种间的杂交研究；②杂交时必须考虑种的系统关系学并以派内杂交为主导方向；③对我国冷杉属树种进行开花结实习性研究；④开展我国冷杉属树种的系统学和遗传多样性研究；⑤结合现代生物技术和分子生物学知识，克服常规杂交中的困难。

Aimed at the arising problems such as the cross-incompatibility and hybrid's sterility during distant hybridization of fruit trees, effects of different treatments on cross-compatibility of distant hybridization in stone fruits were studied based on the former studies, then physiological and biochemical changes during hybridization between plum and apricot were primarily discussed, and rescue and culture system of hybrids' embryos were also conducted, further the genetic variation and principle between the hybrids and their parents were analyzed and methods of identification of hybrids were established.

针对果树远缘杂交过程中存在的杂交不亲和性及杂种不育性等问题，在前人研究的基础上，研究了不同处理对核果类果树远缘杂交亲和性的效应，并探讨了李、杏属间杂交过程中发生的生理生化变化以及花粉与柱头的互作，建立了李、杏属间远缘杂种的胚培养技术体系，建立了杂种鉴定检测方法，并分析了杂种后代与其父母本之间的遗传变异及规律，最终将一批远缘杂交新种质定植于大田，同时对自然界存在的杏梅种质资源进行了RAPD分析。

Brief probe hybridized with long target DNA, the hybridization position had no effects on the binding amount, but mid-position of hybridization would prolong the hybridization time, which was due to the steric hindrance on the surface.

结合位点不同对杂交量无明显影响，但由于晶体表面DNA空间结构的影响，结合位点位于靶序列中间时杂交时间明显延长。

Discovery of these microbial information in these unique ecosystems is rather encouraging for further novel species discovery, exploration and conservation of useful microbes. In the first section, the microbiota diversity of the former salterns was investigated by denaturing gradient gel electrophoresis and fluorescence in situ hybridization.

本研究第一部份先以变性梯度胶体电泳(Denaturing gradient gel electrophoresis，DGGE)针对微生物的16S rDNA片段进行分群，再以生物多样性指数加以描述，另辅以萤光原位杂交技术(Fluorescence in situ hybridization，FISH)调查本区域之微生物多样性。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐