查询词典 hybridization

After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法，并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原，在96孔板中的免疫反应结束后，利用稀硝酸将反应产物分解，然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

Methods The DIO and DIO R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化方法检测下丘脑弓状核神经肽Y的 mRNA和蛋白表达情况。

Methods The DIO and DIO-R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化为法检测下丘脑弓状核神经肽Y的mRNA和蛋白表达情况。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

The decomposition of chromosome was a main problem in construction of BAC library. DNA discompose would be avoided or decreased by adjustments of component and pH of chromosome extraction buffer or pre-electrophoresis for 6-8h before digestion. Identification of inter-species hybrids was a necessary step for creating a new germplasm. Two samples were selected from offspring of hybridization and backcross of a cross between cabbage and Chinese cabbage.

鉴定种间杂种是利用远缘杂交创造新种质时的一项重要工作，本研究运用细胞核DNA含量流式测定技术，对甘蓝和白菜杂交及回交各世代的DNA含量进行检测，以监控遗传物质在不同材料间的流向，并从回交二代植株群体中筛选了两个材料，具有和白菜差异不显著的DNA含量，可推断其为核质杂种。

Fluorescent in situ hybridization can be used to evaluate the distribution and function of bacterial population in situ.

荧光原位杂交技术也可以用来评价在situ上的细菌的分布以及功能。

METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot.

采用基于PCR和消减杂交的基因克隆技术构建体外培养的人PDLF与GF差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的克隆，并进行测序和计算机分析。

The paper is concerned about a teaching and learning reform of hybridization experiment of Drosophila melanogaster.

本文介绍关于黑腹果蝇杂交实验的一次教学改革尝试。

It was showed that from a super-high yield germplasm HS Z6-06-56 derivated from an interspecific hybridization between common wheat and durum wheat: Dates of flagging,heading and maturing were 1,1 and 2 d earlier compared with its accepter′s parent of Shi 4185,respectively,there were no differences between traits of plant height,head length,spikelets/head by means of anova analysis.

通过对普通小麦、四倍体硬粒小麦种间杂交超高产新种质HS Z6-06-56性状表现分析表明：该种质与受体亲本石4185相比，挑旗期、抽穗期及成熟期分别提早1，1及2 d，株高、穗长、小穗数三性状等同于受体。

To determine whether the same molecular mechanisms are inoled in the formation process, the expression pattern of transcription factors regulating endoderm and gut deelopment in the mouse embryo was examined by in situ hybridization and compared with in io expression.

为了证明在成形过程中是否也具有相似的分子机制，我们通过原位杂交同体内表达比对的方法来检查小鼠胚胎中调节内胚层和肠道发育的转录因子的表达模式。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。