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The second half inspects SciTech from the four angles of the wholeness of the system of human nature: from the angle of self, as the production of making use of objects by self, SciTech can be in favor of self or dissever self, which, the author thinks, is little helpful to the consolidation and transcendence of self; from the angle of individuality, for its aim is to eliminate individuality and to pursue commonness, there is distinct conflict between SciTech and individuality; from the angle of valueness, only have been the means of affirming and realizing the value of human nature and the purpose of personality, can SciTech have internal legitimacy; from the angle of sublimatity, human's right and rational needs make SciTech impel development of human nature healthfully, lust which is the distortion of needs make the trap, and technologizing the human nature will never be the way and aim of sublimating human nature.

下部分从人性系统整体性的四个角度考察;从自我看,科技作为自我"假于外物"的产物而推动或肢解自我,对自我的整合和提升少有实质性作用;从个性看,它是销匿个性而寻求共性的结果,与个性之间矛盾突出;从价值性看,它只有成为确证和实现人性价值和人格目标的手段,才具有内在合法性;从提升性看,人的正当合理的需要使科技推动人性健康发展,欲望对需要的扭曲造成陷阱,使人性技术化不是人性提升的途径和目标。

As to this has formed different understanding between developed country and developing country, the developed country thinks human rights has already substituted " foundation stone " that the traditional sovereign equality has become the international relations, emphasize the sovereignty is to threaten human rights , if wanting to protect human rights , must limit state sovereignty , is it advocate " human rights higher than sovereignty "," human rights borderless ", for maintain human rights can go on " humanistic intervention " to other country to do one's utmost.

对此在发达国家和发展中国家之间形成了不同的认识,发达国家认为人权已经替代了传统的主权平等成为国际关系的"基石",强调主权就是威胁人权,要保护人权就必须限制国家主权,极力宣扬"人权高于主权"、"人权无国界",为了维护人权可以对他国进行"人道主义干预"。

The doctrine and practice of the Council of Europe in the promotion and protection of has had profound influence on the international protection of human rights as follows: Regional approach is the effective way to enforce the UD ;The international institutions of human rights must upgrade the justiciability of human rights through their reformations; States ought to exercise their sovereignty actively in protecting human rights in regional mechanism . The consciousness of the subject of individual right is naturally required to be strengthened in the protection of human rights; The international institutions of human rights ought to improve their working efficiency.

内容提要欧洲理事会采取集体办法促进和保护人权的理论和实践对建立和完善国际人权保护制度产生了深远的影响:区域性人权保护办法是在区域层面上施行《世界人权宣言》的有效途径;国际人权机构应通过改革不断提高人权的可司法性;主权国家应在采取集体办法保护区域人权方面积极行使主权权利;人权国际保护内在地要求增强个人的权利主体意识;国际人权保护机构应不断提高其工作效率。

Meanwhile, a calculating formula of the net flow is provided through the analysis, showing that the flow has no concern with the application itself, but has concern with the operating speed of keyboard and mouse At present, two versions have been realized in Human-to-Human system, the first version, being a similar-platform Human-to-Human system, is applied to Windows 98/NT; another version is realized on Heterogeneous type operating systems as Unix, Linux, Windows 98, Windows NT, while the Human-to-Human Interaction systems used on micro-interaction facilities as HPC are still under the research.

目前,HHII系统已实现两种版本,一个版本是同平台人-人交互系统,适用于Windows98/NT平台,另一版本是在Unix,Linux,Windows98,WindowsNT等异构型操作系统上实现,对于HPC等微型交互设备上人-人交互系统正在研究之中。

This text human life state problem root visual field in human life meaning the inside, Increase actual in the demand of contemporary human life state, with the human life"s important function that China with the west already the cognition of the some human life state is a foundation to shine on, pass to analysis human life state innate character with study, announce to public the human life state to path, combine as to it"s the exaltation proceeds studying of first step.

本文将人生境界问题植根于人生意义的视野中,立足于引导和提升当代人人生境界立场,以中国和西方已有的人生境界的理论认识为基础和参照,通过对人生境界本质的分析和研究,揭示了人生境界对人生的重要作用,并对其提高的途径进行了初步的探讨。全文共分为三大部分:第一部分主要阐述人生境界的本质。

Article 2 This law applies to the drafting of laws, regulations and rules relating to the protection of human rights within the borders of the People's Republic of China, the furnishing of various conditions for the realization of human rights by way of administrative measures, the realization of judicial remedies against acts violating human rights, human rights propaganda and human rights education within the whole society, the performance of Signatory States' obligations under human rights treaties that have been already concluded or acceded to by the People's Republic of China, the conduct of international exchange and co-operation in the field of human rights and other activities.

第二条 在中华人民共和国境内制定有关人权保障的法律、法规和规章,通过行政管理措施来为人权的实现提供各种条件,对侵害人权的行为实现司法救济,在全社会进行人权宣传和人权教育,根据中华人民共和国已经缔结和参加的国际人权公约履行公约下的缔约国应尽义务,进行人权领域的国际交流和合作等活动,适用本法。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

And Marx insisted on using "human"s thinking method" to understand the relation between human being and nature from beginning to end. He proceeded from natura instincts of human life to study human being and nature alternately. He also went further into the real relation between human being and nature in the human"s practice. From his points, nature is the humans nature, the relation between human being and nature is the social relation of people.

而且,马克思在理解人与自然关系的过程始终坚持&人的思维方式&,他从人的生命本性出发来看待交互中的人与自然,在人的实践活动中探讨人与自然的真实关系;在他看来,自然是人的自然,人与自然的关系即人与人的社会关系。

Abstract] objective for the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.methods aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with ph 4, human immunoglobulin and human hepatitis b immunoglobulin for intravenous injection with ph 4/pasteurization,treatment on human fibrinogen and human coagulation factor ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.the mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.results human albumin pasteurization inactivated vsv and sindbis virus,hrig low ph inactivated vsv and sindbis virus,hrig low ph inactivated hiv virus,ivig low ph/pasteurization inactivated hiv virus,ivig low ph inactivated hiv virus,hbig low ph/pasteurization inactivated vsv,sindbis and polio virus.conclusion suggestion about improvement on unreasonable parameter and standard of virus inactivation is made,cultivate target virus directly from final product by appropriate cell or method to monitoring viral safety of blood plasma product is advanced.

目的 分析血液制品特定灭活因子的时效动力学曲线,科学性地评价与改进不合理的病毒灭活参数。方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒,人血白蛋白采用巴氏消毒法,狂犬病人免疫球蛋白采用低ph常温孵放法,静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低ph/巴氏消毒法;人纤维蛋白原、人凝血因子ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料,统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数,制作灭活病毒动力曲线图并进行分析。结果人血白蛋白巴氏灭活vsv与sindbis病毒,hrig低ph灭活vsv和sindbis病毒,hrig低ph灭活hiv病毒,ivig低ph/巴氏灭活hiv病毒,ivig低ph灭活hiv病毒,hbig低ph/巴氏灭活vsv、sindbis与polio病毒。结论建议改进病毒灭活验证标准与不合理的灭活参数;采用终产品样品通过合适细胞或方法直接培养目标病毒来有效监测血液制品的病毒安全性。

From human angles people can understand the human nature and analyze the various basis and characteristic of creating individualized aesthetic perception. The new-type aesthetic perception can make people appreciate beautiful things in the way of human. It helps create the ideal relationship between nature, human, self-essence and human, and improve the enrichment and perfection of human nature. Thus realize the really beautiful human nature.

接下来就分析建构&个性化&审美观的多元基础及其特征,并对全文做出总结,所建构的新型的审美观将使人真正能够按照&人&的方式去进行审美,有利于建立人与自然、人与人、人与自身本质之间的理想关系,并推动人性的丰富与完善,实现真正&美&的人性。

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