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He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

People who sleep six to seven hours anight live longer, and those who sleep eight hours or more dieyounger, according to the latest study everconducted on the subject.

与人们的普遍观念恰恰相反，就睡眠所做的最新研究发现每晚睡6-7个小时的人要比睡8个小时或更长时间的人寿命长。

The index case was a 5-month-old infant girl who had anisocoria from 4 weeks of age. One hour after 1% apraclonidine hydrochloride was instilled in each eye, there was blanching of the periocular skin, and the parents reported that the infant was excessively drowsy. Two hours after receiving apraclonidine, the infant was taken to the emergency department for lethargy and unresponsiveness, with bradycardia (84 beats/min), shallow respiration (40 breaths/min), and blood pressure 115/85 mm Hg. Oxygen saturation was 80% but improved rapidly to 100% when oxygen was given via a face mask. Eight hours after receiving apraclonidine, the infant had returned to normal.

指标案例是一名5个月大的女婴，她从4周大时就有瞳孔大小不等的状况；在1%的apraclonidine氯化氢徐徐滴点她的双眼后1个小时，眼眶周边的皮肤即变白，而她的家长表示她变得非常嗜睡；在使用apraclonidine 2个小时之后，该名女婴因嗜睡和无应答性，以及心搏过慢（每分钟84下）、呼吸浅弱（每分钟40次）以及血压115/85 mm Hg等症候而被送进急诊室；血氧饱和度原先为80%，在面罩给氧后迅速回复到100%，在使用apraclonidine 8个小时后，该名女婴终于回复健康状态。

The index case was a 5-month-old infant girl who had anisocoria from 4 weeks of age. One hour after 1% apraclonidine hydrochloride was instilled in each eye, there was blanching of the periocular skin, and the parents reported that the infant was excessively drowsy. Two hours after receiving apraclonidine, the infant was taken to the emergency department for lethargy and unresponsiveness, with bradycardia (84 beats/min), shallow respiration (40 breaths/min), and blood pressure 115/85 mm Hg. Oxygen saturation was 80% but improved rapidly to 100% when oxygen was given via a face mask. Eight hours after receiving apraclonidine, the infant had returned to normal.

指标案例是一名5个月大的女婴，她从4周大时就有瞳孔大小不等的状况；在1%的apraclonidine氯化氢徐徐滴点她的双眼后1个小时，眼眶周边的皮肤即变白，而她的家长表示她变得非常嗜睡；在使用apraclonidine 2个小时之后，该名女婴因嗜睡和无应答性，以及心搏过慢（每分钟84下）、呼吸浅弱（每分钟40次）以及血压115/85 mm Hg等症候而被送进急诊室；血氧饱和度原先为80%，在面罩给氧后迅速回复到100%，在使用apraclonidine 8个小时后，该名女婴终於回复健康状态。

The percent of atresic follicular increase with dosage rising (P.05).The result of expression of Bax and Bcl-2 showed that the Bax expressed widely in every groups, and it enhanced with dosage, there is extreme significant difference between experiment group and control group (P.01),there is not difference between withdrawal 48 hours and 96 hours.

免疫组化法检测Bax和Bcl-2蛋白表达，结果Bax在各组普遍表达，并随镉剂量的加大而表达增强，实验组与对照组间差异极显著(P.01)，48h与96h两时间段间无差异；Bcl-2则普遍表达较弱或不表达，各组间无显著性差异。

Zhaoyuan is the well-known "Gold Capital in China" and it has abundant tourism resources, such as "Luoshan National Forest Park","Jindu-Hotspring City","Gold Museum" and Coastal Resort. The Binhai New Area owns highly developed speedway network and has possessed all the conditions for autocamp: it is 3 hours' drive to Provincial Capital and 5 hours' drive to Beijing and Tianjin Area

招远市滨海新区位于环渤海经济圈内，海水清澈、环境优美、气候宜人，是理想的旅游度假胜地；招远市是知名的&中国金都&，&罗山国家森林公园&、&金都-汤城&、&黄金博物馆&、滨海度假等旅游资源丰富；区内高速公路网完善，省内旅游实现3个小时车程，距京津地区5个小时的车程，具备建设汽车营地的各种条件。

The experiment is very hard to remove phosphor. The best backwash cycle is 6 hours and use air water backwashing. The treatment efficiency could come back to 70% after 12 hours behind the backwash.(5)The removal rate of double-layer medium BAF is better.

5与单一炉渣填料的生物滤池的处理效果相比，本试验的双层填料曝气生物滤池显著提高了出水水质，尤其提高了滤池对NH_3-N的处理效果：滤池对COD_的去除率平均提高了10％，对NH_3-N的去除率平均提高了将近30％。

Dan Rink, Personal Career Coach, feels thirty or thirty-five Hours is about right, and Barbra recommends "at least 20 hours/week for a serious job search."

丹冰场，个人职业生涯教练，认为30或35小时左右正确的，芭芭拉建议&至少20小时/周进行认真的寻找工作。&

So, if you have a job that requires you to be functioning early in the morning hours, bartending into the wee hours would probably not be a wise choice.

如果你的工作要求你早晨很早工作，那么在凌晨做调酒工作大概就不是明智的选择。

Methods Hep G2 cells were treated with Aroclor1254,DDT and Toxaphene respectively for 24 hours,and further incubated in the medium supplemented with cytochalasin B(3?μg/ml)for 24 hours.Micronucleiwere scored in 1?000 binucleated Hep G2 cells.

方法人肝肿瘤细胞Hep G2经Aroclor1254、毒杀芬和滴滴涕染毒24 h，继续在补充细胞松弛素B(3μg/ml)的培养液中培养24 h后，计数1 000个双核细胞中的微核。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。