查询词典 homology

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

In comparison with the reported corresponding sequences, the putative amino acid sequence shares high homology with assimilatory nitrate reductase of other microalgae, with identity 78% to Dunaliella tertiolecta, 60% to Volvox carteri, 58% to Chlamydomonas reinhardtii, and 50% to Chlorella vulgaris, respectively.

2.3 杜氏盐藻NR基因原核表达载体在大肠杆菌中的表达和功能鉴定(1)pMAL-NR-2组大肠杆菌SDS-PAGE结果显示：在140kDa左右出现一条特异性条带，分子量与MBP-NR融合蛋白相符（BMP+NR=42.5kDa+99.5kDa=142kDa），在IPTG（0.15mmol/L）37℃诱导时，该蛋白量占全菌蛋白的21.06％；对照组无此特异带。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后，利用体外结合的方法发现，蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力，尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段，也能明显地结合于酵母核骨架，这一结果表明二者的SAR顺序具有一定的同源性，为骨架结合区域具有进化保守性提供了又一证据。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods Using epidemiological investigation method to cultivate bacilli and detect the homology.

采用现场流行病学调查方法，对标本进行细菌培养和菌株同源性检测。

BLASTX analysis revealed that 47﹪ of the ESTs displayed the significant homology to functionally known or unknown genes from GenBank database. tBLASTx analysis suggested that 12 unisequences showed significant similarity to dbEST entries, of which 9 unisequences were from cDNA libraries of other biotrophic basidiomycetes.

BLASTx分析表明，47﹪的ESTs与GenBank中报道的功能已知或未知蛋白具有相似性。tBLASTx分析表明12个uniseqs与EST数据库中的序列具有相似性，其中9个是来自担子菌的cDNA文库。

The partial N-terminal amino acid sequences of LccA and LccB were AIGPK and GIGPV, which show high homology to those of the laccases of other white-rot basidiomycetes.

用SDS-PAGE测定两漆酶的分子量分别为64.2ku和60.7ku；LccA和LccB的最适作用温度分别是40℃和50℃；最适反应pH值为3.5和4.0；其N-末端部分氨基酸序列为AIGPK和GIGPV，与其他真菌漆酶相比显示很高的同源性，以ABTS为底物的Km值分别为68.96μmol／L和76.92μmol／L。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

Sequence analysis of IL-2 showed that it had high homology with that of bubalus bubalis, except the difference of post-translational modification.

序列分析表明，林麝IL-2及其受体结合域与水牛的高度保守，而在翻译后修饰的预测活性位点上和水牛的有所不同。

The homology tree established by DNAMAN showed the variation among the 14 varieties of Canarium album.

使用DNAMAN软件建立的同源关系树说明了不同橄榄品种的变异程度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。