查询词典 homological

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。