查询词典 homogenate

Methods:Pigspleen cells were cultured with HBvaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator;the homogenate was diluted with distilled water to 1∶1, then frozen and melt six times at25 ℃ before placed in the dialyzer at the melting state , after that dialyzed with 1∶4 distilled water as the outdialyzate at 4 ℃ for 48 h;the final outdialyzate was collected as the HBVSTF.

采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1∶1稀释，－25 ℃反复冻融6次，自来水融化后装入透析袋中，用1∶4的蒸馏水作透析外液，于4 ℃透析48 h，透析液即为乙肝病毒特异性转移因子。

Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法；采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1：1稀释，-25℃反复冻融6次，自来水融化后装入透析袋中，用1：4的蒸馏水作透析外液，于4℃透析48h，透析液即为乙肝病毒特异性转移因子。

It was helpful to extract enzyme by using homogenate in citrate-phosphate buffer; The enzyme activity extracted from pH5.6 buffer is higher than that of pH5.8.The loss of enzyme activity of this zymin is better, but easily used in manufacture.

丙酮粉提取法会引起回收酶活性较大损失，其总酶活仅是缓冲液匀浆法和浸提法的1/5、1/7，但酶制剂本身的酶活性较好，使用方便，有利于工业化生产应用。

To establish anaplasma artificial infection model in experimental animals which treated with dexamethasone to investigate the transmission route of the pathogen, mice were infected via intra-abdominal and subcutaneous inoculation with the blood from infected cattle, the homogenate of infected gadflies and viscera of infected mice, contacting infected mice, respectively, while rabbits were infected through co-feeding with affected cattle.

采用地塞米松注射液长期抑制试验动物的免疫力，通过小鼠腹腔、皮下接种含有无浆体病原的奶牛血液、阳性厩蝇内容物、脏器悬液，并进行阳性小鼠接触感染和兔子临床同层感染等试验，研究奶牛无浆体的感染和传播及在试验动物体内的消长规律，建立起无浆体人工感染模型，调查临床中病原体的实际传播途径。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得据经统计学处理差异无显著性。

38 patients with nasal polyp and 15 control cases were chosen. Their IL-5 concentration in tissue homogenate were measured by enzyme-linked immunosorbent assay. All samples were stained with Chromotrope 2R histochemical and SP immunohistochemical method separately to examine eosinophil and IgE+ cells in nasal polyp.

方法采用免疫组化SP法检测38例鼻息肉，15例正常鼻腔粘膜组织中IgE阳性细胞表达情况；采用酶联免疫吸附试验检测以上病例中IL-5含量；采用Chromotrope 2R特染方法检测以上病例中Eos浸润情况。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

The RNA genome of virus used forRT-PCR can be isolated not only from the infected chicken embryonated fluids, but also directly from tissue homogenate of field samples. The detection results of 67 field samples were identical with that of the conventional diagnosis.

本方法只需一个RT-PCR反应，整个过程在8小时内完成，既可取感染鸡胚尿囊液检测， 2002年）丫H人《'WIf寸它义：也可从病料直接检测。

Methods:The EAE in Lewis rats was induced by giving hypodermal injections in feet with spinal cords homogenate of guinea pigs; The histological changes were observed under light microscope, then changes of incidence rate、latency、clinical symptoms and histology of EAE which were effected by idazoxan with different doses were compared by method of quantization.

采用豚鼠全脊髓匀浆脚垫皮下注射，免疫Lewis大鼠复制EAE模型，采用量化评分法，比较不同剂量组咪唑克生对EAE发病率、潜伏期、临床症状和组织学的影响。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。