查询词典 hog-wild

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

A renegate hog grunts in the undergrowth.

一只野猪在灌木丛中咕哝。

For the pork purveyors and hog farmers who make up the nation's $15 billion pork industry, that's a disaster.

对于猪肉供应商和养猪的农民来说，他们为国家创造150亿美元的。对于猪肉行业，这是一个灾难。

This circulation made energy and material used reasonably and basically, increased output, reduced waste disposal and improves economical benefits.The comparison and analysis on economical benefits of "four-in-one" model household who use standard draft and Ordinary greenhouse big awning household who do not use standard draft showed that after using standard draft, The methane-generating pit, the hog house and the greenhouse were integrated in together, farmers income significantly increased and economic evaluation indicator improved too.

这样的循环关系使能量和物质基本都得到了合理利用，增加了产出，减少了废物的排放，提高了经济效益。7、对采用标准草案的&四位一体&农户和未采用标准草案的普通大棚农户的经济效益比较分析显示，采用标准(来源：bf0fAf0BC论文网www.abclunwen.com)草案的用户农户收入显著提高，经济效益评价指标亦有所改善。

Develop Hog, Poultry feed business, dealer or farm, provide service accordingly, maintain customer relationship

开发猪料、禽料的经销商、用户，大型牧场；提供相应的服务；维护客户关系

With 5 years Hog, Poultry feed sales or farm service working experience, also 2 years team leader experience is preferred

1年以上工作经验 5年以上畜禽料销售或农场技术服务工作经验，2年以上团队带领经验

This additional step with the bakery meal further dilutes the MC concentrations in poultry feed as compared to that in hog feed.

这额外的步骤与膳食烘烤进一步稀释浓度的三菱商事在家禽饲料相比，在猪饲料。

The facts: on Sunday, August 12, this year of our Lord, Eunice tried to kill me with her papas Civil War sword and Olivia Ann cut up all over the place with a fourteen-inch hog knife.

事实情况是：公园纪年的本年八月十二日，那天是礼拜天，尤尼斯企图用其父曾在内战时期用过的剑杀死我，奥莉维亚-安用14英寸长的杀猪刀把这里砍的伤痕累累。

For C/N of hog manure is lower than that of straw, quick-acting nitrogen in soil of the treatment applied M F was immobilized less than that applied C or W F.

因此，有机肥与化肥配施的各个处理在玉米拔节期，必需适量追肥，以满足玉米生育后期生殖生长的需求。

The wolf, the snake, the hog, not wanting in me.

豺狼毒蛇和蠢猪的脾气，我心中并不缺少

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。