查询词典 histochemical

38 patients with nasal polyp and 15 control cases were chosen. Their IL-5 concentration in tissue homogenate were measured by enzyme-linked immunosorbent assay. All samples were stained with Chromotrope 2R histochemical and SP immunohistochemical method separately to examine eosinophil and IgE+ cells in nasal polyp.

方法采用免疫组化SP法检测38例鼻息肉，15例正常鼻腔粘膜组织中IgE阳性细胞表达情况；采用酶联免疫吸附试验检测以上病例中IL-5含量；采用Chromotrope 2R特染方法检测以上病例中Eos浸润情况。

HSP 70 mRNA expression in the posterior cingulate cortex was detected by using semi-quantitative RT-PCR technique; HSP 70 protein expression in posterior cingulated cortex was determined by immuno-histochemical method.

于用药后24 h，大鼠断头取脑，用半定量RT-PCR技术和免疫组织化学方法检测各组HSP70 mRNA和HSP 70蛋白在大鼠后扣带回皮质区的表达。

Methods: Integrated optical density was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL.

采用ABC免疫组织化学和原位杂交组织化学技术，结合计算机图像处理，测定其积分光密度值。

After fixation,the brain was cut coronally into four blockes:the middle partof cerebrum,midbrain,pons and medulla oblongata.These blockes were embeddedroutingly and serial 8μm sections were prepared for HE,specific histochemical andimmunohistochemical stainings.

所有脑组织经固定后冠状切取大脑中部、中脑、桥脑及延髓4个组织块，常规包埋，连续切片（8μm）分别作HE、组化及免疫组化染色。

The results of histochemical GUS assay showed that no expression of GUSint was observed in Agrobacterium, with transient expression as high as 100% in cotyledonary node of Brassica napus L. after inoculated for three days. It not only verified that the vector was successfully constructed, bur also laid a basis for further increasing the stable transformation efficiency of other Brassica plants, and rapidly clarifying the function and pattern of expression and regulation of a unknown gene.

组织化学染色结果表明，GUSint在农杆菌中没有表达，而在接种3d的油菜中可高效瞬间表达，其中在子叶柄中瞬间表达率高达100%，这一方面证实载体构建成功，另一方面也为进一步优化油菜及其它芸薹属植物转化体系及在油菜中快速研究目的基因的功能和表达调控模式奠定了基础。

Then histochemical, electron microscopic, enzyme histo cytochemical and confocal laser scanning microscopic methods were employed to study the distribution of vinculin, cathepsin Dand nematolysosomes ...

结果在正常对照组神经元，组织蛋白酶D与纽蛋白分布于胞质及突起内；在CD及PMA处理神经元，纽蛋白及组织蛋白酶D的分布呈向心性移动，但集聚的部位不同；电镜酶细胞化学方法显示CD组及PMA组神经元内线状溶酶体均增多。

METHODS The mechanism and condition of thrombus formation by copper needle were studied in vivo and in vitro using electrophysics, atom absorption spectrophtometry, histological, and histochemical methods.

以体外实验和大鼠体内实验为模型，用电子物理学、原子光谱吸收法、组织学和组织化学等方法研究铜针通电后血栓形成的机制和条件。

Effects of medication , electropuncture and integrated electropuncture and medication treatments on BDNF in the CA1 and CA3 of hippocampus of the rat depression model were observed and compared by using immuno - histochemical techniques.

以免疫组化技术显示比较电针结合药物、电针、药物对慢性应激抑郁模型大鼠海马CA1、CA3区BDNF的影响。

Methods Rat spinal cord injury models were established by aneurysm clip compression, and nestin expression and proliferation of ependymal cells at different times were shown with pathological and immuno-histochemical staining.

应用动脉瘤夹压迫建立大鼠脊髓损伤模型，通过组织病理学及免疫组织化学方法检测不同时段室管膜细胞的反应性增生和神经外胚层多潜能细胞特异性抗原nestin的表达。

INTERVENTIONS: Embryonal 14-day SD retina was transplantedin-between the epithalamus and hypothalamus of the midbrain in P1 rat and the right eye of the P1 rat was removed simultaneously. The development time of TP on the 9th day after operation was corresponding to the normal retina on the 1st day after born, which was then recorded as TP1, and so on. The development and differentiation was shown by histochemical method.

干预：用胚14 d SD大鼠视网膜移植至P1大鼠中脑左侧上、下丘间，同时摘除P1鼠右眼，术后第9天移植视网膜的发育时间与生后第1天正常视网膜相当，记为TP1，余类推，应用组织化学方法显示神经元的发育和分化。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。