查询词典 hippocastanum

Inconclution, for produce Aesculus hippocastanum L in a mass, we should induce secondary somatic embryos with integral somatic embryos, the suitable culture medium is MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L.

综合上述研究结果，为大规模生产欧洲七叶树，以完整的体细胞胚诱导次生胚效果最好，合适的培养基为MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L。

The medium of 1/2MS+ 0.4mg/L NAA +0,2mg/L IBA was used for rooting, and the rooting rate was 75%. The origin and development of somatic embryos was observed by histological method. It was found that the somatic embryos of Aesculus hippocastanum L were developed from single cell.

通过组织细胞学方法研究体细胞胚的起源与发生部位，发现欧洲七叶树体细胞胚为单细胞起源，外植体的表面及内部都可以发生体细胞胚。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。