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ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.
针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状,即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况,本研究从筛选玉米转基因工程所需要的优良受体入手,采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法,以及现代分子标记定位主效QTL分析方法,对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究,取得以下主要研究结果:(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合,在相同培养条件下,对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定,发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数,但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系,有的基因型,如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异;有的基因型,如自交系综31,仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599,但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距,仅为19-599和18-599的1/3左右;有的基因型,如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低,平均仅在3%左右,而且胚性愈伤组织绿苗发生数表现也很差,基本上没有分化成苗。
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And that set the tone for a parade of glowering tough cookies who looked like they'd stepped straight out of Gangs of New York . Kohl-eyed, clutching their silver-topped canes like cudgels, they stormed down the catwalk in tailored finery. It was a typical McQueen scenario: immaculately realized garments underpinned by a hint of horror movie (a leather butcher's apron transmogrified into Rollerball rig) and the promise of rough sex (muscles that stopped at nothing, for no one).
呼应著服装主题,所有男模们都装扮成Gangs of New York电影中气势强悍的凶狠角色:中东妇女惯用的墨黑粗线眼妆、手持镀银棍棒长杖、并身著合身长大衣;这是McQueen最擅长的戏剧化主题,运用或惊悚或暴力的电影概念与剧情,来勾勒建构出设计师意欲传递的非主流思维。
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When human dignity is whored away and there is no self-respect to be found; when we awake one day to find that we are completely at the mercy of those who are devoid of human emotions and feelings because they have been de-sensitized to any hint of morality - then, maybe we will wish we had NOT kept our ears and eyes and mouths shut.
当人的尊严是whored以外,也没有自我尊重被发现,当有一天,我们清醒地发现,我们完全任凭谁是这些人缺乏情感和情绪,因为他们已经去认识到任何暗示的道德-那么,也许我们会想,我们没有使我们的耳朵和眼睛,闭嘴。
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Early in 1995, in his "Desire" series where red and blue lines come in strong contrast with each other, Ma Yiping instinctively used his personal experience to drop a hint of the transcendence of life. From 1996 to 2001, his "Images" series established the development direction of his art, owing to its artistic expression_r_r of overloaded psychological priori images. In his works before or after 2003, dream-like symbolic images presented perplexed feeling of human beings about the universe and the physical world. In his "images" series in 2006, uncertain images stood on black cube-shaped objects and ambiguous edges pointed to various uncertain directions with the accompaniment of silver light and shadow. At this point, the intuitive perception of the essence of nature made his artistic style gradually take shape. In his later "fetish" series, in which he gradually became mature in spiritual and symbolic expression_r_rs, Ma Yiping fully grasped the eternal sense of the spiritual world.
在早期,1995年的《欲望》马一平用对比强烈的红色、蓝色层层涂盖、本能地运用自身感受以暗示的手法表达对生命的超越。1996-2001年以物象为主题的系列作品对超载心理先验物象的表达方式确定了他艺术作品的发展方向,2003年前后的作品中,梦一般的暗示性物体程现了生命本体对宇宙物理世界的茫然,在2006年的《物象》系列中,黑色的方体上不确定的物状、模糊的边缘在泛着银色光影的衬托下,泛生着各种不确定的指向感,此时对本质直观的感知性的把握使作品艺术风格基本形成,到后来的《神物》已经充分地把握了精神世界里的永恒感,画面中所能达到的精神性和神密暗示意义的表现手法已近成熟。
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This is from a better time, when men were men, movie titles told you exactly what to expect (hint: an adventure that is fantastic), and Raquel Welch in a catsuit was the closest thing to pornography a man could get without having to go to a seedy-looking theater with sticky floors and Travis Bickle types making gun fingers at the screen.
这是从一个更美好的时候,男人是男性,电影告诉你什么期望(提示:这是一个梦幻般的冒险)和拉奎尔韦尔奇在catsuit是最接近色情男人可以不必去一个邋遢的前瞻性剧院粘性层和特拉维斯Bickle类型使枪的手指在屏幕上。
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She answered that she had to raise a same query with me to abbot about the sentence on her lot actually, and abbot was explaining to her that the Chinese adage of "Make still further progress while made another step at the top of a rod hundred feet high" originally for arouse people's enthusiasm who were studying Buddha in Ancient China, when their merits and virtues would achieve the top, they have to "Make still further progress",but the deep hint meaning is that "You have to be prudent ", it is for advising people to be modest and prudent while their enterprise had made far-gone (Wow, so that is!).
她说其实她也有同样的疑问问庙里的住持,住持告诉她成语&百尺竿头,更进一步&最初是为了鼓励学佛的人即将功德圆满的时候,一定要&更进一步&,但是更深层隐含的意义是&不可不慎&,就是为了劝告人们当他们的事业达到一定的高度的时候一定要谨慎从事。
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Nor was there any hint of the stirring recent fightbacks at Everton and Wigan Athletic.
而且也毫无最近对阵埃佛顿和维根时那种激动人心的反击场面。
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Data from existing X-ray satellites that show images of gas whizzing about black holes at close to the speed of light hint that time slows as the gas plunges into the zone from which escape is impossible.
从现有的X射线卫星传回的数据显示,气体以接近光速的速度呼啸着被吸入黑洞,这暗示当气体进入这个不可逃逸的空间时,时间慢了下来。
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If nitrogen is present as single atoms, it would hint that the diamonds were produced rapidly and at relatively low temperature - as might happen if the diamonds formed from pockets of graphite in the zircons in a single, rapid event. That would make the diamonds young.
如果氮元素被证实是以单个原子的形式存在,这意味着钻石是在相对低温的环境下快速形成的——对那些由锆石中的石墨矿囊转变成形的钻石来说,如果单一而快速的条件具备,这种情况就很可能会发生,并将证明这些钻石的年代并不久远。
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If nitrogen is present as single atoms, it would hint that the diamonds were produced rapidly and at relatively low temperature - as might happen if the diamonds formed from pockets of graphite in the zircons in a single, rapid event.
如果氮元素以单个原子存在,这将暗示这些钻石是在相对比较低温的情况下快速形成的。
- 推荐网络例句
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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.
47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。
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Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.
提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。
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Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...
嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。